Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes.
Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes.
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DOI：
10.1186/gb-2007-8-5-r87
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发表时间：
2007
期刊：
影响因子：
12.3
通讯作者：
Neyfakh AA
中科院分区：
文献类型：
--
作者：
Smith LK;Gomez MJ;Shatalin KY;Lee H;Neyfakh AA
Monitoring of gene knockouts is a new microarray-based genetic technique used for genome-wide identification of conditionally essential genes in bacteria We have developed a new microarray-based genetic technique, named MGK (Monitoring of Gene Knockouts), for genome-wide identification of conditionally essential genes. MGK identified bacterial genes that are critical for fitness in the absence of aromatic amino acids, and was further applied to identify genes whose inactivation causes bacterial cell death upon exposure to the bacteriostatic antibiotic chloramphenicol. Our findings suggest that MGK can serve as a robust tool in functional genomics studies.
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发表时间：
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