The fission yeast mitotic regulator win1+ encodes an MAP kinase kinase kinase that phosphorylates and activates Wis1 MAP kinase kinase in response to high osmolarity.
The fission yeast mitotic regulator win1+ encodes an MAP kinase kinase kinase that phosphorylates and activates Wis1 MAP kinase kinase in response to high osmolarity.
复制标题
裂殖酵母有丝分裂调节因子 win1 编码 MAP 激酶激酶，该激酶可磷酸化并激活 Wis1 MAP 激酶激酶以响应高渗透压。
DOI：
--
复制
发表时间：
1998
影响因子：
3.3
通讯作者：
P. Fantes
中科院分区：
文献类型：
--
作者：
I. Samejima;Shaun Mackie;E. Warbrick;R. Weisman;P. Fantes
Schizosaccharomyces pombe win1-1突变体在细胞周期的G2-M转换中存在缺陷。虽然该缺陷受到wis1+和wis4+的抑制，而wis1+和wis4+是连接应激反应和细胞周期控制的应激激活MAP激酶途径的组成部分，但Win1的分子身份尚不清楚。我们在这里证明了win1+编码1436个残基的多肽，其表观分子大小为180 kDa，并证明win1是一种MAP激酶激酶激酶，可以磷酸化并激活Wis1。尽管Win1和Wis4之间有广泛的相似性，但这两种MAP激酶具有不同的功能。Wis4仅能在非胁迫条件下补偿Win1的损失，以维持基本的Wis1活性，但它不能抑制Win1突变带来的渗透信号缺陷。win1-1突变是16个核苷酸的自发复制，导致移码并产生缺乏激酶结构域的截断蛋白。我们讨论了win1-1 cdc25-22 wee1-50突变体的细胞周期表型及其受wis基因的抑制。
The Schizosaccharomyces pombe win1-1 mutant has a defect in the G2-M transition of the cell cycle. Although the defect is suppressed by wis1+ and wis4+, which are components of a stress-activated MAP kinase pathway that links stress response and cell cycle control, the molecular identity of Win1 has not been known. We show here that win1+ encodes a polypeptide of 1436 residues with an apparent molecular size of 180 kDa and demonstrate that Win1 is a MAP kinase kinase kinase that phosphorylates and activates Wis1. Despite extensive similarities between Win1 and Wis4, the two MAP kinase kinase kinases have distinct functions. Wis4 is able to compensate for loss of Win1 only under unstressed conditions to maintain basal Wis1 activity, but it fails to suppress the osmosignaling defect conferred by win1 mutations. The win1-1 mutation is a spontaneous duplication of 16 nucleotides, which leads to a frameshift and production of a truncated protein lacking the kinase domain. We discuss the cell cycle phenotype of the win1-1 cdc25-22 wee1-50 mutant and its suppression by wis genes.
影响因子：
56.9
作者：
HAN, J;LEE, JD;ULEVITCH, RJ
通讯作者：
ULEVITCH, RJ
影响因子：
56.9
作者：
Posas, F;Saito, H
通讯作者：
Saito, H
影响因子：
56.9
作者：
Peng, CY;Graves, PR;PiwnicaWorms, H
通讯作者：
PiwnicaWorms, H