Nuclear factor-kappa B localization and function within intrauterine tissues from term and preterm labor and cultured fetal membranes.
Nuclear factor-kappa B localization and function within intrauterine tissues from term and preterm labor and cultured fetal membranes.
复制标题
DOI：
10.1186/1477-7827-8-8
复制
发表时间：
2010-01-25
期刊：
影响因子：
--
通讯作者：
Ackerman WE 4th
中科院分区：
文献类型：
--
作者：
Vora S;Abbas A;Kim CJ;Summerfield TL;Kusanovic JP;Iams JD;Romero R;Kniss DA;Ackerman WE 4th
本研究的目的是定量研究p65的核定位和DNA结合活性。p65是核转录因子-kappaB的主要反式激活亚单位，在足月或早产时，在全层胎膜和子宫肌层中的定位和DNA结合活性。来自以下队列的配对全层FM和子宫肌层标本：早产无分娩(PNL，N=22)、自发早产(PTL，N=21)、足月无分娩(TNL，N=23)和自然足月分娩(STL，N=21)。用免疫组织化学方法检测NF-kappaB p65的定位，用酶联免疫吸附试验(ELISA)检测DNA结合活性。无论临床情况如何，核p65标记在羊膜和绒毛膜中很少见。在蜕膜中，相对于TNL组，STL组的核p65标记更多，但在TNL组、PTL组和PNL组之间没有差异。在子宫肌层，弥漫性p65核标记与足月和早产显著相关。在没有或有足月分娩的情况下，羊膜、绒毛蜕膜和子宫肌层标本中基于酶联免疫吸附试验的p65结合活性没有显著差异。然而，使用培养的足月胎膜进行的平行实验显示，即使在没有细胞因子刺激的情况下，也有高水平的p65样结合，这表明这种检测方法在应用于组织标本时可能价值有限。这些结果表明，蜕膜是胎膜中核因子-kappaB调节的重要部位，胞浆隔离以外的机制可能限制足月前核因子-kappaB的激活。
The objective of this study was to quantify the nuclear localization and DNA binding activity of p65, the major transactivating nuclear factor-kappa B (NF-kappaB) subunit, in full-thickness fetal membranes (FM) and myometrium in the absence or presence of term or preterm labor. Paired full-thickness FM and myometrial samples were collected from women in the following cohorts: preterm no labor (PNL, N = 22), spontaneous preterm labor (PTL, N = 21), term no labor (TNL, N = 23), and spontaneous term labor (STL, N = 21). NF-kappaB p65 localization was assessed by immunohistochemistry, and DNA binding activity was evaluated using an enzyme-linked immunosorbent assay (ELISA)-based method. Nuclear p65 labeling was rare in amnion and chorion, irrespective of clinical context. In decidua, nuclear p65 labeling was greater in the STL group relative to the TNL cohort, but there were no differences among the TNL, PTL, and PNL cohorts. In myometrium, diffuse p65 nuclear labeling was significantly associated with both term and preterm labor. There were no significant differences in ELISA-based p65 binding activity in amnion, choriodecidual, and myometrial specimens in the absence or presence of term labor. However, parallel experiments using cultured term fetal membranes demonstrated high levels of p65-like binding even the absence of cytokine stimulation, suggesting that this assay may be of limited value when applied to tissue specimens. These results suggest that the decidua is an important site of NF-kappaB regulation in fetal membranes, and that mechanisms other than cytoplasmic sequestration may limit NF-kappaB activation prior to term.
登录
查看更多内容
DOI：
10.1016/j.jsbmb.2004.12.027
发表时间：
2005-02-01
影响因子：
4.1
作者：
Mendelson, CR;Condon, JC
通讯作者：
Condon, JC
影响因子：
5.8
作者：
Chapman, NR;Europe-Finner, GN;Robson, SC
通讯作者：
Robson, SC
影响因子：
4
作者：
Mitchell, C. M.;Johnson, R. F.;Zakar, T.
通讯作者：
Zakar, T.
影响因子：
3.8
作者：
Lappas, M.;Rice, G. E.
通讯作者：
Rice, G. E.
影响因子：
2.9
作者：
Merlino, Amy;Welsh, Toni;Mesiano, Sam
通讯作者：
Mesiano, Sam