SET DOMAIN GROUP 721 protein functions in saline-alkaline stress tolerance in the model rice variety Kitaake.
SET DOMAIN GROUP 721 protein functions in saline-alkaline stress tolerance in the model rice variety Kitaake.
复制标题
SET结构域第721组蛋白在水稻模式品种Kitaake的盐碱胁迫耐受性中起作用。
DOI：
10.1111/pbi.13683
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发表时间：
2021-12
影响因子：
13.8
通讯作者：
Xu ZY
中科院分区：
文献类型：
--
作者：
Liu Y;Chen X;Xue S;Quan T;Cui D;Han L;Cong W;Li M;Yun DJ;Liu B;Xu ZY
为了分离耐盐碱基因位点，我们以模式水稻（Oryza sativa L.）品种北明。其中一个激活标记的插入系，激活标记7（AC 7），显示出对盐碱胁迫的耐受性增加。该表型由编码具有H3 K4甲基转移酶活性的SET结构域组721蛋白的基因的过表达引起。过量表达OsSDG 721的转基因植株表现出耐盐碱表型，沿着叶片倾角增大、抽穗期提前和成熟期提前。相比之下，ossdg 721功能丧失突变体表现出对盐碱胁迫的敏感性增加，其特征在于存活率降低，株高、籽粒大小、粒重和叶角减少。对野生型Kitaake和ossdg 721突变体的RNA测序（RNA-seq）分析表明，OsSDG 721正调控高亲和力钾（K+）转运蛋白1;5（OsHKT 1;5）的表达水平，OsHKT 1; 5编码在盐胁迫下维持K+/Na+稳态的Na+选择性转运蛋白。此外，我们发现OsSDG 721结合并沉积在OsHKT 1;5的启动子和编码区的H3 K4 me 3标记，从而在盐碱胁迫下上调OsHKT 1;5的表达。总的来说，通过产生Kitaake激活标签库，我们确定了H3 K4甲基转移酶OsSDG 721增强了水稻的盐碱胁迫耐受性。
To isolate the genetic locus responsible for saline–alkaline stress tolerance, we developed a high‐throughput activation tagging‐based T‐DNA insertion mutagenesis method using the model rice (Oryza sativa L.) variety Kitaake. One of the activation‐tagged insertion lines, activation tagging 7 (AC7), showed increased tolerance to saline–alkaline stress. This phenotype resulted from the overexpression of a gene that encodes a SET DOMAIN GROUP 721 protein with H3K4 methyltransferase activity. Transgenic plants overexpressing OsSDG721 showed saline–alkaline stress‐tolerant phenotypes, along with increased leaf angle, advanced heading and ripening dates. By contrast, ossdg721 loss‐of‐function mutants showed increased sensitivity to saline–alkaline stress characterized by decreased survival rates and reduction in plant height, grain size, grain weight and leaf angle. RNA sequencing (RNA‐seq) analysis of wild‐type Kitaake and ossdg721 mutants indicated that OsSDG721 positively regulates the expression level of HIGH‐AFFINITY POTASSIUM (K+ ) TRANSPORTER1;5 (OsHKT1;5), which encodes a Na+‐selective transporter that maintains K+/Na+ homeostasis under salt stress. Furthermore, we showed that OsSDG721 binds to and deposits the H3K4me3 mark in the promoter and coding region of OsHKT1;5, thereby upregulating OsHKT1;5 expression under saline–alkaline stress. Overall, by generating Kitaake activation‐tagging pools, we established that the H3K4 methyltransferase OsSDG721 enhances saline–alkaline stress tolerance in rice.
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