Clinical significance of SOX9 in human non-small cell lung cancer progression and overall patient survival.
Clinical significance of SOX9 in human non-small cell lung cancer progression and overall patient survival.
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SOX9 在人类非小细胞肺癌进展和患者总体生存中的临床意义
DOI：
10.1186/1756-9966-31-18
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发表时间：
2012-03-03
期刊：
影响因子：
--
通讯作者：
Gong LY
中科院分区：
文献类型：
--
作者：
Zhou CH;Ye LP;Ye SX;Li Y;Zhang XY;Xu XY;Gong LY
性别决定区Y(SRY)相关的高迁移率组盒9(SOX9)是发育所必需的重要转录因子，调节相关途径中靶基因的表达。本研究旨在探讨SOX9在人非小细胞肺癌(NSCLC)中的表达及其与非小细胞肺癌发生发展的关系。方法采用Western blotting和实时荧光定量逆转录聚合酶链式反应(RT-PCR)方法检测正常肺细胞、肺癌细胞系、8对配对肺癌组织及癌旁正常肺组织中SOX9蛋白和mRNA的表达。应用免疫组织化学方法检测142例非小细胞肺癌组织中SOX9蛋白的表达。结果肺癌细胞株中SOX9在mRNA和蛋白水平均有上调，在非小细胞肺癌组织中SOX9的mRNA和蛋白水平也高于相应的癌旁非癌肺组织。免疫组织化学分析显示，在142例石蜡包埋的肺癌组织中，有74例(52.1%)SOX9高表达。统计学分析显示，sOX9表达上调与非小细胞肺癌的组织学分期显著相关(P=0.017)，且sOX9表达水平高的患者生存期短(P<；0.001)。多因素分析表明，SOX9表达上调可能是非小细胞肺癌患者生存的独立预后指标。结论SOX9可作为一种新的非小细胞肺癌预后指标，在NSCLC的发生、发展过程中起一定作用。
BackgroundSex determining region Y (SRY)-related high mobility groupbox 9 (SOX9) is an important transcription factor required for development, which regulates the expression of target genes in the associated pathway. The aim of this study was to describe the expression of SOX9 in human non-small cell lung cancer (NSCLC) and to investigate the association between SOX9 expression and progression of NSCLC.MethodsSOX9 protein and mRNA expression in normal human pneumonocytes, lung cancer cell lines, and eight pairs of matched lung cancer tissues and their adjacent normal lung tissues were detected by Western blotting and real-time reverse transcription-polymerase chain reaction (RT-PCR). Immunohistochemistry was used to determine SOX9 protein expression in 142 cases of histologically characterized NSCLC. Statistical analyses were applied to test for prognostic and diagnostic associations.ResultsSOX9 in lung cancer cell lines was upregulated at both mRNA and protein levels, and SOX9 mRNA and protein were also elevated in NSCLC tissues compared with levels in corresponding adjacent non-cancerous lung tissues. Immunohistochemical analysis demonstrated a high expression of SOX9 in 74/142 (52.1%) paraffin-embedded archival lung cancer biopsies. Statistical analysis indicated that upregulation of SOX9 was significantly correlated with the histological stage of NSCLC (P= 0.017) and that patients with a high SOX9 level exhibited a shorter survival time (P< 0.001). Multivariate analysis illustrated that SOX9 upregulation might be an independent prognostic indicator for the survival of patients with NSCLC.ConclusionsThis work shows that SOX9 may serve as a novel and prognostic marker for NSCLC, and play a role during the development and progression of the disease.
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