Herpes simplex virus type 1 ICP27 protein: Its expression, purification and specific antiserum production
Herpes simplex virus type 1 ICP27 protein: Its expression, purification and specific antiserum production
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单纯疱疹病毒1型ICP27蛋白：其表达、纯化和特异性抗血清的产生
DOI：
10.1007/s12250-010-3116-2
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发表时间：
2010
影响因子：
5.5
通讯作者：
A. C. Zheng
中科院分区：
文献类型：
--
作者：
Lei Zhao;X. Ren;A. C. Zheng
单纯疱疹病毒1型（HSV-1）是唇疱疹和其他更严重疾病的病原体。HSV-1感染细胞蛋白27（ICP 27）是一种与目前在所有疱疹病毒中鉴定的基因产物同源的即刻早期调节磷蛋白。为了制备抗ICP 27的抗血清，进一步研究其生物学功能，将ICP 27基因克隆到pET-28 a（+）载体中，在大肠杆菌中表达ICP 27蛋白。coli中表达，经Ni ~（2+）-NTA亲和树脂柱纯化，制备抗血清。Western blot分析表明，该抗血清可识别重组蛋白，免疫荧光试验（IFA）显示，该抗血清可特异性地检测HSV-1感染细胞中的ICP 27。因此，特异性抗血清将为进一步研究ICP 27在HSV-1感染中的生物学功能提供有价值的工具。
Herpes simplex virus type 1 (HSV-1) is the causative agent of cold sores and other more serious diseases. HSV-1 infected-cell protein 27 (ICP27) is an immediate-early regulatory phosphoprotein homologous to gene products identified in all classes of herpesviruses so far. To raise the antiserum to ICP27 for further characterization of its biological function, the ICP27 gene was cloned into the pET-28a (+) vector, then ICP27 protein was expressed in E. coli and purified by nickel-nitrilotriacetic acid (Ni2+-NTA) affinity resin column, finally the purified protein was used to raise antiserum. Western blot analysis demonstrated that the antiserum recognized the recombinant protein, and the antiserum was able to probe the ICP27 in HSV-1 infected cells with high specificity by immunofluorescence assay (IFA). Therefore, the specific antiserum will provide a valuable tool for further studies investigating ICP27’s biological function during HSV-1 infection.
影响因子：
10.5
作者：
Sandri-Goldin, RM
通讯作者：
Sandri-Goldin, RM
影响因子：
3.7
作者：
Johnson, Karen E.;Song, Byeongwoon;Knipe, David M.
通讯作者：
Knipe, David M.