Parallel astrocyte calcium signaling modulates olfactory bulb responses.
Parallel astrocyte calcium signaling modulates olfactory bulb responses.
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DOI：
10.1002/jnr.24634
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发表时间：
2020-08
影响因子：
4.2
通讯作者：
Deneen B
中科院分区：
文献类型：
--
作者：
Ung K;Tepe B;Pekarek B;Arenkiel BR;Deneen B
星形胶质细胞是中枢神经系统中含量最丰富的神经胶质细胞。它们通过各种机制调节突触功能，但对于整体神经元回路功能的研究相对较少。利用小鼠嗅觉系统的易操纵性，我们操纵了星形胶质细胞的活动，并研究了星形胶质细胞如何调节嗅球反应。为此，我们从基因上定位了星形胶质细胞和神经元，以便在体内广域成像钙离子对气味刺激的反应。我们发现，星形胶质细胞的气味反应图与兴奋性神经元活动重叠。通过利用化学遗传学来控制星形胶质细胞中的钙离子活性，我们发现气味诱发的神经元活动是相互影响的，这表明星形胶质细胞的激活抑制了神经元的气味反应。随后，行为学实验显示，星形胶质细胞的操纵同时影响气味检测阈值和辨别能力，这表明星形胶质细胞在嗅觉处理回路中发挥着积极的作用。总之，这些研究表明，星形胶质细胞钙信号通过调节感觉回路来影响嗅觉行为。
Astrocytes are the most abundant glial cell in the CNS. They modulate synaptic function through a variety of mechanisms, and yet remain relatively understudied with respect to overall neuronal circuit function. Exploiting the tractability of the mouse olfactory system, we manipulated astrocyte activity and examined how astrocytes modulate olfactory bulb responses. Towards this, we genetically targeted both astrocytes and neurons for in vivo wide-field imaging of Ca2+ responses to odor stimuli. We found that astrocytes exhibited odor-response maps that overlap with excitatory neuronal activity. By manipulating Ca2+ activity in astrocytes using chemical genetics we found that odor-evoked neuronal activity was reciprocally affected, suggesting that astrocyte activation inhibits neuronal odor responses. Subsequently, behavioral experiments revealed that astrocyte manipulations affect both odor detection threshold and discrimination, suggesting that astrocytes play an active role in olfactory sensory processing circuits. Together, these studies show that astrocyte calcium signaling contributes to olfactory behavior through modulation of sensory circuits.
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DOI：
10.1073/pnas.0700293104
发表时间：
2007-03-20
影响因子：
11.1
作者：
Armbruster, Blaine N.;Li, Xiang;Roth, Bryan L.
通讯作者：
Roth, Bryan L.
影响因子：
16.2
作者：
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Kuner T
影响因子：
56.9
作者：
Bindocci, Erika;Savtchouk, Iaroslav;Volterra, Andrea
通讯作者：
Volterra, Andrea
影响因子：
6.2
作者：
Durkee, Caitlin A.;Covelo, Ana;Araque, Alfonso
通讯作者：
Araque, Alfonso
影响因子：
6.2
作者：
Hanson E;Armbruster M;Cantu D;Andresen L;Taylor A;Danbolt NC;Dulla CG
通讯作者：
Dulla CG