Cationic nanoparticles induce nanoscale disruption in living cell plasma membranes.
Cationic nanoparticles induce nanoscale disruption in living cell plasma membranes.
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DOI：
10.1021/jp9033936
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发表时间：
2009-08-13
期刊：
影响因子：
--
通讯作者：
Orr BG
中科院分区：
文献类型：
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作者：
Chen J;Hessler JA;Putchakayala K;Panama BK;Khan DP;Hong S;Mullen DG;Dimaggio SC;Som A;Tew GN;Lopatin AN;Baker JR;Holl MM;Orr BG
长期以来，人们已经认识到阳离子纳米颗粒诱导细胞膜渗透性。最近，已经发现阳离子纳米颗粒诱导支撑的脂质双层中纳米级孔的形成和/或生长。在本文中，我们表明，非细胞毒性浓度的阳离子纳米粒子诱导30-2000 pA电流在293 A和KB细胞，符合纳米级缺陷，如一个单一的孔或一组孔在细胞膜上的总面积从1到350 nm 2。其他形式的纳米级缺陷，包括吸附到脂质双层上或嵌入脂质双层中的纳米颗粒穿孔剂也是一致的;尽管与水通道相比，缺陷的尺寸必须增加以解释离子传导的任何降低。单个缺陷形成事件需要1 - 100 ms，而膜重新密封可能发生数十秒。膜片钳数据为活细胞膜中纳米级缺陷的形成提供了直接证据。阳离子聚合物的数据进行比较和对比膜片钳数据获得的AMO-3，一个小分子，提出使定义明确的3.4 nm的孔在脂质双层。在这里，我们观察到的数据与AMO-3在活细胞膜中制造~3 nm孔一致。
It has long been recognized that cationic nanoparticles induce cell membrane permeability. Recently, it has been found that cationic nanoparticles induce the formation and/or growth of nanoscale holes in supported lipid bilayers. In this paper we show that non-cytotoxic concentrations of cationic nanoparticles induce 30–2000 pA currents in 293A and KB cells, consistent with a nanoscale defect such as a single hole or group of holes in the cell membrane ranging from 1 to 350 nm2 in total area. Other forms of nanoscale defects, including the nanoparticle porating agents adsorbing onto or intercalating into the lipid bilayer are also consistent; although the size of the defect must increase to account for any reduction in ion conduction, as compared to a water channel. An individual defect forming event takes 1 – 100 ms, while membrane resealing may occur over tens of seconds. Patch-clamp data provide direct evidence for the formation of nanoscale defects in living cell membranes. The cationic polymer data are compared and contrasted with patch-clamp data obtained for AMO-3, a small molecule that is proposed to make well-defined 3.4 nm holes in lipid bilayers. Here, we observe data that are consistent with AMO-3 making ~3 nm holes in living cell membranes.
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DOI：
10.1021/jp8013783
发表时间：
2008-08-07
期刊：
The journal of physical chemistry. B
影响因子：
--
作者：
Kelly CV;Leroueil PR;Orr BG;Banaszak Holl MM;Andricioaei I
通讯作者：
Andricioaei I
影响因子：
15
作者：
Yang, Lihua;Gordon, Vernita D.;Wong, Gerard C. L.
通讯作者：
Wong, Gerard C. L.
影响因子：
3.1
作者：
Noronha, FSM;Cruz, JS;Horta, MF
通讯作者：
Horta, MF
DOI：
10.1021/jp801377a
发表时间：
2008-08-07
期刊：
The journal of physical chemistry. B
影响因子：
--
作者：
Kelly CV;Leroueil PR;Nett EK;Wereszczynski JM;Baker JR Jr;Orr BG;Banaszak Holl MM;Andricioaei I
通讯作者：
Andricioaei I
影响因子：
10.4
作者：
通讯作者：
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