Lipopolysaccharide-induced expression of nitric oxide synthase II in the guinea pig vestibular end organ
Lipopolysaccharide-induced expression of nitric oxide synthase II in the guinea pig vestibular end organ
复制标题
脂多糖诱导豚鼠前庭终末器官中一氧化氮合酶 II 的表达
DOI：
10.1007/s004050050040
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发表时间：
1998
影响因子：
2.6
通讯作者：
M. Anniko
中科院分区：
文献类型：
--
作者：
M. Takumida;M. Anniko
为探讨豚鼠前庭器官接种细菌脂多糖(LPS)是否可诱导一氧化氮合酶(NOS)II的形成，动物经鼓室注射1 mg LPS 48h后，可观察到不同程度的热量反应受损，前庭毛细胞也有类似的变性。这些作用可被一氧化氮合酶竞争性抑制剂N-硝基-L-精氨酸甲酯阻断。结果表明，正常情况下在豚鼠前庭器官中检测不到的NOS II在接种内毒素后存在，它产生了一氧化氮，作为导致细胞损伤的毒性因素。如果是真的，内毒素可能是研究内耳疾病前庭发病机制的一种可重复性的方法。
Abstract The purpose of the investigation was to ascertain whether inoculation of bacterial lipopolysaccharide (LPS) into the vestibular organ of the guinea pig might induce formation of nitric oxide synthase (NOS) II. Forty-eight hours after the animals were injected with 1 mg transtympanic LPS, varying degrees of impaired caloric responses were observed with similar degeneration of vestibular hair cells. These effects could be blocked with N-nitro-l-arginine methylester, a competitive inhibitor of NOS. Findings suggested that NOS II, which was not normally detectable in the guinea pig vestibular organ but was present following inoculation of LPS, produced the nitric oxide as the toxic factor causing cell damage. If true, LPS may represent a reproducible method for studying the vestibular pathogenesis of inner ear disease.
DOI：
10.1073/pnas.88.14.6368
发表时间：
1991-07-01
影响因子：
11.1
作者：
DAWSON, VL;DAWSON, TM;SNYDER, SH
通讯作者：
SNYDER, SH
DOI：
10.1172/jci116439
发表时间：
1993-05
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
B. Markewitz;J. Michael;D. Kohan
通讯作者：
B. Markewitz;J. Michael;D. Kohan
DOI：
10.1073/pnas.90.7.3024
发表时间：
1993-04-01
影响因子：
11.1
作者：
KOPROWSKI, H;ZHENG, YM;DIETZSCHOLD, B
通讯作者：
DIETZSCHOLD, B