Peripheral shift in the viable chondrocyte population of the medial femoral condyle after anterior cruciate ligament injury in the porcine knee.
Peripheral shift in the viable chondrocyte population of the medial femoral condyle after anterior cruciate ligament injury in the porcine knee.
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DOI：
10.1371/journal.pone.0256765
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Fleming BC
中科院分区：
文献类型：
--
作者：
Costa MQ;Murray MM;Sieker JT;Karamchedu NP;Proffen BL;Fleming BC
前交叉韧带损伤导致膝关节内侧间室的创伤后骨关节炎，即使在手术治疗后。在这个过程的早期，关节软骨内的软骨细胞分布如何变化目前尚不清楚。本研究的目的是在临床前模型中研究前交叉韧带横断后股骨内侧髁内的软骨细胞分布。将42只青少年尤卡坦小型猪分配接受单侧前交叉韧带手术（n = 36）或不手术（n = 6）。在手术后1周和4周获得内侧股骨髁的中央冠状切片，并通过全载玻片成像和ImageJ中使用的细胞计数批量处理工具测量软骨细胞分布。Ki-67免疫组织化学染色鉴定增殖细胞。空陷窝、核溶解、核碎裂和核固缩用于鉴定不可逆细胞损伤的区域。不可逆细胞损伤的平均面积在完整对照中为0%，在损伤后1周为13.4%（95%置信区间：6.4，20.3），在损伤后4周为19.3%（9.7，28.9）（p <0.015）。这些区域最靠近股骨关节内切迹。含有活软骨细胞的剩余区域具有Ki-67阳性细胞（p <0.02），并且在中间区（p <0.03）和深区（p = 0.001）中细胞密度增加。对于整个切片，术后软骨细胞总数没有显著变化;然而，相对于完整对照组，损伤后1周和4周，内侧股骨髁周边区域的细胞密度显著增加（分别为p = 0.032和0.004）。这些数据表明，前交叉韧带损伤后，股骨内侧髁的活软骨细胞群的外周转移，并进一步表明，具有增殖能力的软骨细胞并不局限于一个特定的软骨层。
Anterior cruciate ligament injuries result in posttraumatic osteoarthritis in the medial compartment of the knee, even after surgical treatment. How the chondrocyte distribution within the articular cartilage changes early in this process is currently unknown. The study objective was to investigate the chondrocyte distribution within the medial femoral condyle after an anterior cruciate ligament transection in a preclinical model. Forty-two adolescent Yucatan minipigs were allocated to receive unilateral anterior cruciate ligament surgery (n = 36) or no surgery (n = 6). Central coronal sections of the medial femoral condyle were obtained at 1- and 4 weeks after surgery, and the chondrocyte distribution was measured via whole slide imaging and a cell counting batch processing tool utilized in ImageJ. Ki-67 immunohistochemistry was performed to identify proliferating cells. Empty lacunae, karyolysis, karyorrhexis, and pyknosis were used to identify areas of irreversible cell injury. The mean area of irreversible cell injury was 0% in the intact controls, 13.4% (95% confidence interval: 6.4, 20.3) at 1-week post-injury and 19.3% (9.7, 28.9) at 4 weeks post-injury (p < .015). These areas occurred closest to the femoral intra-articular notch. The remaining areas containing viable chondrocytes had Ki-67-positive cells (p < .02) and increased cell density in the middle (p < .03) and deep zones (p = .001). For the entire section, the total chondrocyte number did not change significantly post-operatively; however, the density of cells in the peripheral regions of the medial femoral condyle increased significantly at 1- and 4 weeks post-injury relative to the intact control groups (p = .032 and .004, respectively). These data demonstrate a peripheral shift in the viable chondrocyte population of the medial femoral condyle after anterior cruciate ligament injury and further suggest that chondrocytes with the capacity to proliferate are not confined to one particular cartilage layer.
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DOI：
10.2217/ijr.10.3
发表时间：
2010-04
期刊：
International journal of clinical rheumatology
影响因子：
--
作者：
Grogan SP;D'Lima DD
通讯作者：
D'Lima DD
DOI：
10.1177/0363546521989265
发表时间：
2021-03
期刊：
The American journal of sports medicine
影响因子：
--
作者：
Karamchedu NP;Murray MM;Sieker JT;Proffen BL;Portilla G;Costa MQ;Molino J;Fleming BC
通讯作者：
Fleming BC
影响因子：
3.3
作者：
Sophia Fox AJ;Bedi A;Rodeo SA
通讯作者：
Rodeo SA
影响因子：
2.4
作者：
Okafor, Eziamaka C.;Utturkar, Gangadhar M.;DeFrate, Louis E.
通讯作者：
DeFrate, Louis E.
影响因子：
2.8
作者：
Jablonski, Christina L.;Leonard, Catherine;Krawetz, Roman J.
通讯作者：
Krawetz, Roman J.