Updates to the guinea pig animal model for in-vivo auditory neuroscience in the low-frequency hearing range.
Updates to the guinea pig animal model for in-vivo auditory neuroscience in the low-frequency hearing range.
复制标题
在低频听力范围内，在体内听觉神经科学的豚鼠动物模型中更新。
DOI：
10.1016/j.heares.2022.108603
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发表时间：
2022-10
期刊：
影响因子：
2.8
通讯作者：
Sadagopan, Srivatsun
中科院分区：
文献类型：
--
作者：
Montes-Lourido, Pilar;Kar, Manaswini;Pernia, Marianny;Parida, Satyabrata;Sadagopan, Srivatsun
为了深入了解听觉处理的一般原理，选择其自然行为集包含正在研究的过程的模式生物是至关重要的。这种推理导致了听觉神经科学研究的各种动物模型的发展，如豚鼠，沙鼠，龙猫，兔子和雪貂;但近年来，尖端的分子工具和其他方法在小鼠模型中的可用性导致对这些独特的模型物种的兴趣减弱。随着实验室越来越多地将体内组件纳入其研究计划，将这些现代神经科学工具中的一些应用于非小鼠小动物模型的程序和技术的全面描述将使研究人员能够利用可能最适合测试其特定假设的独特模型物种。在这篇手稿中，我们详细描述了我们开发的方法，将这些工具应用于豚鼠动物模型，以回答有关复杂声音（如发声）的神经处理问题。我们描述了发声采集技术，行为测试，记录听觉脑干反应和频率跟随反应，颅内神经信号，包括局部场电位和单个单位活动，以及转基因的表达，允许光遗传学操纵的神经活动，所有在清醒和头部固定的豚鼠。我们展示了丰富的数据集，可以使用这些技术获得的行为和电生理水平，强调豚鼠作为一个多功能的动物模型，研究复杂的听觉处理。更一般地说，这里描述的方法适用于广泛的小型哺乳动物，使研究人员能够解决特定的听觉处理问题的模式生物，最适合回答他们。
For gaining insight into general principles of auditory processing, it is critical to choose model organisms whose set of natural behaviors encompasses the processes being investigated. This reasoning has led to the development of a variety of animal models for auditory neuroscience research, such as guinea pigs, gerbils, chinchillas, rabbits, and ferrets; but in recent years, the availability of cutting-edge molecular tools and other methodologies in the mouse model have led to waning interest in these unique model species. As laboratories increasingly look to include in-vivo components to their research programs, a comprehensive description of procedures and techniques for applying of some of these modern neuroscience tools to a non-mouse small animal model would enable researchers to leverage unique model species that may be best suited for testing their specific hypotheses. In this manuscript, we describe in detail methods we have developed to apply these tools to the guinea pig animal model to answer questions regarding the neural processing of complex sounds, such as vocalizations. We describe techniques for vocalization acquisition, behavioral testing, recording of auditory brainstem responses and frequency-following responses, intracranial neural signals including local field potential and single unit activity, and the expression of transgenes allowing for optogenetic manipulation of neural activity, all in awake and head-fixed guinea pigs. We demonstrate the rich datasets at the behavioral and electrophysiological levels that can be obtained using these techniques, underscoring the guinea pig as a versatile animal model for studying complex auditory processing. More generally, the methods described here are applicable to a broad range of small mammals, enabling investigators to address specific auditory processing questions in model organisms that are best suited for answering them.
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影响因子：
3
作者：
Drucker CB;Carlson ML;Toda K;DeWind NK;Platt ML
通讯作者：
Platt ML
使用纳米制造的神经探针进行多重、高密度电生理学。
影响因子：
3.7
作者：
Du J;Blanche TJ;Harrison RR;Lester HA;Masmanidis SC
通讯作者：
Masmanidis SC
影响因子：
16.2
作者：
Chambers AR;Resnik J;Yuan Y;Whitton JP;Edge AS;Liberman MC;Polley DB
通讯作者：
Polley DB
影响因子：
3.7
作者：
Atkinson PJ;Wise AK;Flynn BO;Nayagam BA;Richardson RT
通讯作者：
Richardson RT
DOI：
10.1111/ejn.12580
发表时间：
2014-07
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Coomber B;Berger JI;Kowalkowski VL;Shackleton TM;Palmer AR;Wallace MN
通讯作者：
Wallace MN