The effect of dietary zinc--and polyphenols intake on DMBA-induced mammary tumorigenesis in rats.
The effect of dietary zinc--and polyphenols intake on DMBA-induced mammary tumorigenesis in rats.
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DOI：
10.1186/1423-0127-19-43
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发表时间：
2012-04-16
影响因子：
11
通讯作者：
Tokarz A
中科院分区：
文献类型：
--
作者：
Bobrowska-Korczak B;Skrajnowska D;Tokarz A
本研究的目的是研究膳食中补充锌和多酚化合物（即白藜芦醇和染料木素）对化学诱导乳腺癌疗效的影响，以及与正常乳腺组织相比，肿瘤中选定元素（锌、铜、镁、铁、钙）含量的变化。雌性spraguedawley大鼠分为研究组，除标准饮食和DMBA（7,12-二甲基-1,2-苯[a]蒽）外，分别给予锌离子（Zn）或锌离子+白藜芦醇（Zn +白藜芦醇）或锌离子+染料木黄酮（Zn +染料木黄酮）灌食，为期40天至20周龄。采用电感耦合等离子体发射光谱法（ICP-OES）分析了镁、铁、锌、钙等元素。用原子吸收分光光度计测定样品中的铜含量。无论何种饮食（标准、锌、锌+白藜芦醇、锌+染料木素），dba诱导的乳腺癌发生均未被抑制。相反，补充锌+白藜芦醇组的肿瘤发生速度明显快于对照组。在对所选元素进行定量分析的基础上，我们发现，在dmba诱导的乳腺肿瘤中，无论使用何种饮食，铜和铁都大量积累，这两种元素具有强烈的促氧化作用，同时镁含量显著降低。补锌与白藜芦醇的结合导致肿瘤中所研究元素的含量与正常组织中的含量有特别大的差异。饲粮中添加锌和多酚类化合物，即白藜芦醇和染料木素，对降低肿瘤组织中铜的水平和抑制大鼠乳腺癌的发生没有影响。无论应用何种饮食，大鼠肿瘤过程的发展导致癌变组织中铁和镁含量与健康乳腺组织相比发生变化。在膳食中添加锌离子和白藜芦醇可显著提高乳腺癌的癌变率，增加dba诱导的乳腺肿瘤的数量。
The aim of the study was to investigate the effect of dietary supplementation with zinc and polyphenol compounds, i.e. resveratrol and genistein, on the effectiveness of chemically induced mammary cancer and the changes in the content of selected elements (Zn, Cu, Mg, Fe, Ca) in tumors as compared with normal tissue of the mammary gland. Female Sprague-Dawley rats were divided into study groups which, apart from the standard diet and DMBA (7,12-dimethyl-1,2- benz[a]anthracene), were treated with zinc ions (Zn) or zinc ions + resveratrol (Zn + resveratrol) or zinc ions + genistein (Zn + genistein) via gavage for a period from 40 days until 20 weeks of age. The ICP-OES (inductively coupled plasma optical emission spectrometry) technique was used to analyze the following elements: magnesium, iron, zinc and calcium. Copper content in samples was estimated in an atomic absorption spectrophotometer. Regardless of the diet (standard; Zn; Zn + resveratrol; Zn + genistein), DMBA-induced breast carcinogenesis was not inhibited. On the contrary, in the Zn + resveratrol supplemented group, tumorigenesis developed at a considerably faster rate. On the basis of quantitative analysis of selected elements we found - irrespectively of the diet applied - great accumulation of copper and iron, which are strongly prooxidative, with a simultaneous considerable decrease of the magnesium content in DMBA-induced mammary tumors. The combination of zinc supplementation with resveratrol resulted in particularly large differences in the amount of the investigated elements in tumors as compared with their content in normal tissue. Diet supplementation with zinc and polyphenol compounds, i.e. resveratrol and genistein had no effect on the decreased copper level in tumor tissue and inhibited mammary carcinogenesis in the rat. Irrespectively of the applied diet, the development of the neoplastic process in rats resulted in changes of the iron and magnesium content in the cancerous tissue in comparison with the healthy mammary tissue. The application of combined diet supplementation with zinc ions and resveratrol considerably promoted the rate of carcinogenesis and increased the number of DMBA-induced mammary tumors.
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影响因子：
2.6
作者：
Ng, KH;Bradley, DA;Looi, LM
通讯作者：
Looi, LM
影响因子：
7.7
作者：
Alkhalaf, Moussa;El-Mowafy, Abdulla;Al-Attyiah, Rajaa
通讯作者：
Al-Attyiah, Rajaa
影响因子：
4.8
作者：
Bowers, JL;Tyulmenkov, VV;Klinge, CM
通讯作者：
Klinge, CM
影响因子：
5
作者：
Hashemi, Mohammad;Ghavami, Saeid;Los, Marek
通讯作者：
Los, Marek
影响因子：
8.8
作者：
Hilakivi-Clarke, L;Onojafe, I;Raygada, M;Cho, E;Skaar, T;Russo, I;Clarke, R
通讯作者：
Clarke, R