Proteomic analysis of multiple primary cilia reveals a novel mode of ciliary development in mammals.
Proteomic analysis of multiple primary cilia reveals a novel mode of ciliary development in mammals.
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DOI：
10.1242/bio.20121081
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发表时间：
2012-08-15
期刊：
影响因子：
2.4
通讯作者：
Takeda S
中科院分区：
文献类型：
--
作者：
Narita K;Kozuka-Hata H;Nonami Y;Ao-Kondo H;Suzuki T;Nakamura H;Yamakawa K;Oyama M;Inoue T;Takeda S
纤毛是结构和功能多样的细胞器，其功能障碍导致纤毛病。虽然最近的研究已经发现了共同的纤毛运输机制，有限的信息是可用的纤毛蛋白质组，特别是感觉亚型，这可能会提供深入了解其功能和发育障碍。在本研究中，我们进行了蛋白质组学分析的独特，多个9+0纤毛脉络丛上皮细胞（CPECs）。幼猪CPEC纤毛的分析鉴定了868个蛋白质。其中，396个与9+0感光纤毛（外节）的蛋白质组共有，而只有152个与9+2纤毛和鞭毛的蛋白质组共有。各种信号分子在CPEC特异性纤毛亚群中富集，暗示了感觉功能的多样性。纤毛组还包括用于纤毛运动的分子，如RMB 9。在来自幼年猪或成年小鼠的CPEC中，RMB 9定位于纤毛的亚群，而它们是非运动的。小鼠脉络丛活体成像显示新生CPEC纤毛能有力搏动，1-2周内纤毛运动减弱并丧失。新生儿CPEC纤毛的搏动特征是可变的，不同于典型的9+2室管膜纤毛，但Efhc 1介导的机制来调节搏动频率是共享的两种类型的纤毛。值得注意的是，超微结构分析显示，在新生儿CPEC中不仅存在9+0，而且存在9+2和非典型纤毛亚型。总之，这些结果确定了保守和可变的感觉纤毛组件，并证明了一种新的模式纤毛在哺乳动物的发展。
Cilia are structurally and functionally diverse organelles, whose malfunction leads to ciliopathies. While recent studies have uncovered common ciliary transport mechanisms, limited information is available on the proteome of cilia, particularly that of sensory subtypes, which could provide insight into their functional and developmental diversities. In the present study, we performed proteomic analysis of unique, multiple 9+0 cilia in choroid plexus epithelial cells (CPECs). The analysis of juvenile swine CPEC cilia identified 868 proteins. Among them, 396 were shared with the proteome of 9+0 photoreceptor cilia (outer segment), whereas only 152 were shared with the proteome of 9+2 cilia and flagella. Various signaling molecules were enriched in a CPEC-specific ciliome subset, implicating multiplicity of sensory functions. The ciliome also included molecules for ciliary motility such as Rsph9. In CPECs from juvenile swine or adult mouse, Rsph9 was localized to a subpopulation of cilia, whereas they were non-motile. Live imaging of mouse choroid plexus revealed that neonatal CPEC cilia could beat vigorously, and the motility waned and was lost within 1–2 weeks. The beating characteristics of neonatal CPEC cilia were variable and different from those of typical 9+2 cilia of ependyma, yet an Efhc1-mediated mechanism to regulate the beating frequency was shared in both types of cilia. Notably, ultrastructural analysis revealed the presence of not only 9+0 but also 9+2 and atypical ciliary subtypes in neonatal CPEC. Overall, these results identified both conserved and variable components of sensory cilia, and demonstrated a novel mode of ciliary development in mammals.
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DOI：
10.1073/pnas.94.7.3094
发表时间：
1997-04-01
影响因子：
11.1
作者：
Lim, L;Zhou, HP;Costa, RH
通讯作者：
Costa, RH
影响因子：
4.8
作者：
Fukatsu, K;Bannai, H;Mikoshiba, K
通讯作者：
Mikoshiba, K
影响因子：
3.4
作者：
Mayer, Ulrich;Kueller, Alexander;Moehrlen, Frank
通讯作者：
Moehrlen, Frank
影响因子：
64.5
作者：
Gerdes JM;Davis EE;Katsanis N
通讯作者：
Katsanis N
影响因子：
7
作者：
Olsen, JV;de Godoy, LMF;Mann, M
通讯作者：
Mann, M