lncRNA TINCR facilities bladder cancer progression via regulating miR‑7 and mTOR.
lncRNA TINCR facilities bladder cancer progression via regulating miR‑7 and mTOR.
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DOI：
10.3892/mmr.2020.11530
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发表时间：
2020-11
影响因子：
3.4
通讯作者：
Sun L
中科院分区：
文献类型：
--
作者：
Xu G;Yang H;Liu M;Niu J;Chen W;Tan X;Sun L
长非编码RNA（lncRNA）与多种人类恶性肿瘤有关，但含TINCR泛素结构域的lncRNA（TINCR）在膀胱癌中的分子机制仍不清楚。本研究发现，与癌旁正常组织和正常尿路上皮细胞系SV-HUC-1相比，TINCR的表达在膀胱癌组织和细胞系中显着增加。此外，TINCR的高表达与肿瘤转移和肿瘤、淋巴结、转移分期晚期以及膀胱癌患者总生存率降低相关。进一步研究表明，膀胱癌细胞中 microRNA (miR)-7 受到 TINCR 的负介导。沉默TINCR表达显着增加miR-7表达并减少膀胱癌细胞增殖、迁移和侵袭，而敲低miR-7表达则逆转了TINCR下调对膀胱癌细胞的抑制作用。随后mTOR被确定为miR-7在膀胱癌中的靶基因，并且证明mTOR的过度表达逆转了miR-7对膀胱癌细胞的抑制作用。总之，本研究表明 TINCR/miR-7/mTOR 信号传导可能是膀胱癌的潜在治疗靶点。
Long non-coding RNAs (lncRNAs) have been implicated in various human malignancies, but the molecular mechanism of lncRNA TINCR ubiquitin domain containing (TINCR) in bladder cancer remains unclear. The present study found that the expression of TINCR was significantly increased in bladder cancer tissues and cell lines, when compared with that in adjacent normal tissues and normal urinary tract epithelial cell line SV-HUC-1, respectively. Moreover, the high expression of TINCR was associated with tumor metastasis and advanced tumor, node, metastasis stage, as well as reduced overall survival rates of patients with bladder cancer. Further investigation revealed that microRNA (miR)-7 was negatively mediated by TINCR in bladder cancer cells. Silencing of TINCR expression significantly increased miR-7 expression and reduced bladder cancer cell proliferation, migration and invasion, while knockdown of miR-7 expression reversed the inhibitory effects of TINCR downregulation on bladder cancer cells. mTOR was then identified as a target gene of miR-7 in bladder cancer, and it was demonstrated that overexpression of mTOR reversed the inhibitory effects of miR-7 on bladder cancer cells. In conclusion, this study suggests that TINCR/miR-7/mTOR signaling may be a potential therapeutic target for bladder cancer.
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影响因子：
4.6
作者：
Chen Z;Liu Y;He A;Li J;Chen M;Zhan Y;Lin J;Zhuang C;Liu L;Zhao G;Huang W;Cai Z
通讯作者：
Cai Z
miR-7 通过靶向 XRCC2 抑制结直肠癌细胞增殖并诱导细胞凋亡。
影响因子：
4
作者：
Xu K;Chen Z;Qin C;Song X
通讯作者：
Song X
TINCR表达缺失通过激活结直肠癌EpCAM裂解促进增殖和转移
影响因子：
--
作者：
Zhang ZY;Lu YX;Zhang ZY;Chang YY;Zheng L;Yuan L;Zhang F;Hu YH;Zhang WJ;Li XN
通讯作者：
Li XN
影响因子：
3.1
作者：
Zhang JJ;Wang DD;Du CX;Wang Y
通讯作者：
Wang Y
影响因子：
8
作者：
Xu, T-P;Liu, X-X;Shu, Y-Q
通讯作者：
Shu, Y-Q