Comparison of reverse transcription loop-mediated isothermal amplification, conventional PCR and real-time PCR assays for Japanese encephalitis virus.
Comparison of reverse transcription loop-mediated isothermal amplification, conventional PCR and real-time PCR assays for Japanese encephalitis virus.
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日本脑炎病毒的逆转录环介导等温扩增、常规 PCR 和实时 PCR 检测的比较。
DOI：
10.1007/s11033-010-0525-0
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发表时间：
2011-08
影响因子：
2.8
通讯作者：
Yu S
中科院分区：
文献类型：
--
作者：
Chen Z;Liao Y;Ke X;Zhou J;Chen Y;Gao L;Chen Q;Yu S
建立了一种检测乙型脑炎病毒（JEV）的逆转录环介导等温扩增（RT-LAMP）方法。RT-LAMP方法的灵敏度与实时荧光定量RT-PCR方法一致，比常规RT-PCR方法的灵敏度高10倍，检测限为24 copies/μl。该方法特异性强，与登革2型病毒、狂犬病病毒、诺如病毒、星状病毒和人肠道病毒71型均无交叉反应。与常规RT-PCR和real-time RT-PCR相比，RT-LAMP方法在63°C恒温条件下，可在1 h内完成扩增，操作简单，耗时少。结果表明，RT-LAMP方法可作为一种实用的分子诊断工具，用于JEV感染和监测。
We developed and evaluated a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting Japanese encephalitis virus (JEV). The sensitivity of the JEV RT-LAMP assay was in concordance with that of real-time RT-PCR and 10-fold more sensitive than that of conventional RT-PCR, which the detection limit was 24 copies/μl. The JEV RT-LAMP was highly specific, which no cross-reactivity was found with dengue-2 virus, rabies virus, norovirus, astrovirus and human enterovirus 71. The JEV RT-LAMP assay was more simple and less time-consuming compared to the conventional RT-PCR and real-time RT-PCR, which the amplification could be completed in a single tube within 1 h under isothermal conditions at temperature of 63°C. The results suggest that the RT-LAMP assay can be applied as a practical molecular diagnostic tool for JEV infection and surveillance.
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影响因子：
9.4
作者：
Parida, M;Posadas, G;Morita, K
通讯作者：
Morita, K
影响因子：
9.4
作者：
Holbrook, MR;Shope, RE;Barrett, ADT
通讯作者：
Barrett, ADT
影响因子：
3.3
作者：
Nagamine, K;Hase, T;Notomi, T
通讯作者：
Notomi, T
影响因子：
2.1
作者：
Endo, S;Komori, T;Nishimura, K
通讯作者：
Nishimura, K
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3.1
作者：
Santhosh, S. R.;Parida, M. M.;Rao, P. V. Lakshmana
通讯作者：
Rao, P. V. Lakshmana