Green propolis extract attenuates acute kidney injury and lung injury in a rat model of sepsis.
Green propolis extract attenuates acute kidney injury and lung injury in a rat model of sepsis.
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DOI：
10.1038/s41598-021-85124-6
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发表时间：
2021-03-15
影响因子：
4.6
通讯作者：
Andrade L
中科院分区：
文献类型：
--
作者：
Silveira MAD;Capcha JMC;Sanches TR;de Sousa Moreira R;Garnica MS;Shimizu MH;Berretta A;Teles F;Noronha IL;Andrade L
脓毒症是全球急性肾损伤（阿基）和肺损伤的主要原因。尽管治疗进展，脓毒症仍然与高死亡率相关。由于巴西绿色草（GP）具有良好的抗炎、抗氧化和免疫调节特性，我们假设它可以保护盲肠结扎穿孔（CLP）诱导的脓毒症大鼠的肾脏和肺。将雄性Wistar大鼠分为对照组（假手术组）、CLP组（仅CLP组）和CLP + GP组（CLP后6小时用GP治疗），所有大鼠均在手术后6小时接受扩容和抗生素治疗。术后24小时，GP治疗改善了生存率，减弱了脓毒症诱导的阿基，并恢复了肾小管功能。CLP + GP组全血还原型谷胱甘肽水平较高。脓毒症上调肺和肾组织中的Toll样受体4/核因子-κ B轴，以及增加炎性细胞因子水平和巨噬细胞浸润;所有这些作用都被GP减弱。GP治疗减少了肾脏和肺组织中末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记阳性细胞的数量，并保护了肾脏线粒体的形态。我们的数据为GP在脓毒症中的临床试验打开了前景。
Sepsis is the leading cause of acute kidney injury (AKI) and lung injury worldwide. Despite therapeutic advances, sepsis continues to be associated with high mortality. Because Brazilian green propolis (GP) has promising anti-inflammatory, antioxidant, and immunomodulatory properties, we hypothesized that it would protect kidneys and lungs in rats induced to sepsis by cecal ligation and puncture (CLP). Male Wistar rats were divided into groups—control (sham-operated); CLP (CLP only); and CLP + GP (CLP and treatment with GP at 6 h thereafter)—all receiving volume expansion and antibiotic therapy at 6 h after the procedures. By 24 h after the procedures, treatment with GP improved survival, attenuated sepsis-induced AKI, and restored renal tubular function. Whole-blood levels of reduced glutathione were higher in the CLP + GP group. Sepsis upregulated the Toll-like receptor 4/nuclear factor-kappa B axis in lung and renal tissues, as well as increasing inflammatory cytokine levels and macrophage infiltration; all of those effects were attenuated by GP. Treatment with GP decreased the numbers of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling-positive cells in renal and lung tissue, as well as protecting the morphology of the renal mitochondria. Our data open the prospect for clinical trials of the use of GP in sepsis.
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影响因子：
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作者：
Chen L;Deng H;Cui H;Fang J;Zuo Z;Deng J;Li Y;Wang X;Zhao L
通讯作者：
Zhao L
影响因子：
7.2
作者：
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Rafnsson SB
影响因子：
19.6
作者：
Andrade, L;Campos, SB;Seguro, AC
通讯作者：
Seguro, AC
影响因子：
4.6
作者：
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通讯作者：
Rossetti C
DOI：
10.1152/ajprenal.00025.2013
发表时间：
2014-10-15
影响因子：
4.2
作者：
Leelahavanichkul, Asada;Souza, Ana Carolina P.;Yuen, Peter S. T.
通讯作者：
Yuen, Peter S. T.