Mycoplasma biofilms ex vivo and in vivo.
Mycoplasma biofilms ex vivo and in vivo.
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DOI：
10.1111/j.1574-6968.2009.01592.x
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发表时间：
2009-06
影响因子：
2.1
通讯作者：
Dybvig K
中科院分区：
文献类型：
--
作者：
Simmons WL;Dybvig K
生物膜是包裹在聚合物基质中并附着在生物或非生物表面上的微生物群落。尽管它们在体外增强了抵抗抗菌剂和免疫系统组件的能力，但很少有研究在器官水平上讨论生物膜与宿主的相互作用。虽然已经证明支原体在玻璃和塑料表面形成生物膜，但还没有确定它们是否在气管上皮形成生物膜。我们开发了一种气管器官安装系统，允许使用荧光显微镜扫描整个气管内腔表面。我们观察了小鼠呼吸道病原体肺支原体在气管器官培养和实验感染小鼠气管上形成的生物膜，发现其结构和生物学特性与体外形成的生物膜相似。这种气管器官贴壁系统可用于研究呼吸道病原体形成的生物膜与宿主上皮之间的相互作用，并识别体内生物膜形成的因素。
Biofilms are communities of microorganisms that are encased in polymeric matrixes and grow attached to biotic or abiotic surfaces. Despite their enhanced ability to resist antimicrobials and components of the immune system in vitro, few studies have addressed the interactions of biofilms with the host at the organ level. Although mycoplasmas have been shown to form biofilms on glass and plastic surfaces, it has not been determined whether they form biofilms on tracheal epithelium. We developed a tracheal organ mounting system that allowed the entire surface of the tracheal lumen to be scanned by using fluorescence microscopy. We observed the biofilms formed by the murine respiratory pathogen Mycoplasma pulmonis on the epithelium of trachea in tracheal organ culture and in experimentally-infected mice and found similar structure and biological characteristics as biofilms formed in vitro. This tracheal organ mounting system can be used to study interactions between biofilms formed by respiratory pathogens and the host epithelium and to identify the factors that contribute to biofilm formation in vivo.
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影响因子：
3.6
作者：
Gumulak-Smith, J;Teachman, A;Dybvig, K
通讯作者：
Dybvig, K
影响因子：
3.1
作者：
Simmons, WL;Denison, AA;Dybvig, K
通讯作者：
Dybvig, K
影响因子：
3.1
作者：
Simmons, Warren L.;Dybvig, Kevin
通讯作者：
Dybvig, Kevin
影响因子：
3.1
作者：
Simmons, WL;Dybvig, K
通讯作者：
Dybvig, K
影响因子：
3.6
作者：
Bhugra, B;Voelker, LL;Dybvig, K
通讯作者：
Dybvig, K