Age-associated changes in GSH S-transferase gene/proteins in livers of rats
Age-associated changes in GSH S-transferase gene/proteins in livers of rats
复制标题
大鼠肝脏中 GSH S-转移酶基因/蛋白质的年龄相关变化
DOI：
10.1080/13510002.2018.1546985
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发表时间：
2018-01
期刊：
影响因子：
3.8
通讯作者：
Ji Lili
中科院分区：
文献类型：
--
作者：
Xu Shangfu;Hou Dongshun;Liu Jie;Ji Lili
摘要目的：谷胱甘肽S-转移酶（GST）是一类Ⅱ相代谢酶，在药物代谢、抗氧化应激、抗衰老等方面发挥着重要作用。年龄是影响GST表达的关键因素。因此，年龄相关的变化10 GST进行了检查。方法：采集雄性Sprague-Dawley大鼠在胎儿（−2 d）、新生儿（1、7、14和21 d）、青春期（28和35 d）、成年期（60和180 d）和衰老期（540和800 d）时的肝脏，并进行qPCR分析。 提取14、28、60、180、540和800 d的肝脏蛋白，进行Western-blot分析。结果如下：GSTA 1和GSTP 1的表达量随日龄增加而增加，GSTA 4、GSTO 1和GSTZ 1的表达量在成年期逐渐增加，到800日龄时略有下降。GSTM 1、GSTM 3、GSTT 1、GSTT 2和GSTK 1的表达在成年期逐渐增加，在540和800日龄时显著降低。GSTA 1、GSTP 1和GSTZ 1在7-14 d有一个小的峰值。GSTA 1、GSTM 1和GSTP 1的蛋白表达与mRNA表达变化趋势一致。讨论内容：本研究以10种GST在大鼠肝脏发育和衰老过程中的三种表达模式为特征，进一步加深了对GST抗衰老作用的认识。
ABSTRACT Objectives: Glutathione S-transferases (GSTs) are phase-II metabolic enzymes playing important roles in drug metabolism, anti-oxidative stress and anti-aging. Age is a key factor influencing GSTs expression. Thus, age-related changes of 10 GSTs were examined. Methods: Livers from male Sprague–Dawley rats at fetus (−2 d), neonates (1, 7, 14 and 21 d), puberty (28 and 35 d), adulthood (60 and 180 d), and aging (540 and 800 d), were collected and subjected to qPCR analysis. Liver proteins from 14, 28, 60, 180, 540 and 800 d were also extracted for selected protein analysis by Western-blot. Results: The expression of GSTA1 and GSTP1 increased over the life span and the expression of GSTA4, GSTO1 and GSTZ1 gradually increased until adulthood, and slightly decreased at 800 days. The expression of GSTM1, GSTM3, GSTT1, GSTT2 and GSTK1 gradually increased until adulthood, but significantly decreased during aging of 540 and 800 days. There is a small peak at 7–14 d for GSTA1, GSTP1 and GSTZ1. The protein expression of GSTA1, GSTM1 and GSTP1 followed the trend of mRNA changes. Discussion: This study characterized three expression patterns of 10 GSTs during development and aging in rat liver, adding to our understanding of anti-aging role of GSTs.
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雄性大鼠肾脏转运蛋白基因表达与年龄相关的差异
影响因子：
3.4
作者：
Xu Yong-Ji;Wang Yang;Lu Yuan-Fu;Xu Shang-Fu;Wu Qin;Liu Jie
通讯作者：
Liu Jie
影响因子：
5.6
作者：
Schnekenburger M;Karius T;Diederich M
通讯作者：
Diederich M
DOI：
10.1016/j.mito.2008.12.032
发表时间：
2009-02
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
A. L. Shroads;Xu Guo;Vaishali Dixit;Hui-ping Liu;M. James;P. Stacpoole
通讯作者：
A. L. Shroads;Xu Guo;Vaishali Dixit;Hui-ping Liu;M. James;P. Stacpoole
影响因子：
4.1
作者：
B. Németi;M. Poór;Z. Gregus
通讯作者：
B. Németi;M. Poór;Z. Gregus
影响因子：
4.1
作者：
Hubatsch, I;Ridderström, M;Mannervik, B
通讯作者：
Mannervik, B