Phylogenetic diversity of stress signalling pathways in fungi.
Phylogenetic diversity of stress signalling pathways in fungi.
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真菌中应力信号通路的系统发育多样性。
DOI：
10.1186/1471-2148-9-44
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发表时间：
2009-02-21
影响因子：
3.4
通讯作者：
Brown AJ
中科院分区：
文献类型：
--
作者：
Nikolaou E;Agrafioti I;Stumpf M;Quinn J;Stansfield I;Brown AJ
微生物必须感知环境压力、转换这些信号并采取保护性反应才能在恶劣的环境中生存。在这项研究中，我们测试了这样的假设：真菌应激信号通路以独立于系统发育的特定生态位方式快速进化。为了检验这一假设，我们比较了不同真菌物种中应激信号分子的保守性及其抗应激性。这些真菌包括子囊菌、担子菌和微孢子虫，它们占据着从盐碱环境到植物或哺乳动物宿主的高度不同的生态位。真菌对渗透（氯化钠和山梨醇）、氧化（H2O2 和甲萘醌）和细胞壁应激（卡尔科弗洛白和刚果红）的抵抗力表现出显着差异。真菌系统发育与抗逆性之间没有严格的相关性。相反，人类病原体往往对所有三种类型的应激都具有更强的抵抗力，但白色念珠菌对细胞壁应激 Calcoflor White 的敏感性是一个例外。相比之下，植物病原体对氧化应激相对敏感。检查了 18 种真菌物种中渗透、氧化和细胞壁应激信号通路的保守程度。通过执行相互 BLASTP 搜索，鉴定酿酒酵母中功能确定的信号成分的推定直向同源物，并记录这些直向同源物的氨基酸同一性百分比。这表明，一般来说，渗透、氧化和细胞壁应激信号传导途径的中心成分相对保守，而位于这些模块上游的传感器和位于下游的转录调节因子却存在显着差异。胁迫信号通路的保守程度与特定真菌对相应胁迫的抵抗力之间没有明显的相关性。我们的数据与以下假设一致：真菌应激信号成分最近经历了快速进化，以特定生态位的方式调整应激反应。
Microbes must sense environmental stresses, transduce these signals and mount protective responses to survive in hostile environments. In this study we have tested the hypothesis that fungal stress signalling pathways have evolved rapidly in a niche-specific fashion that is independent of phylogeny. To test this hypothesis we have compared the conservation of stress signalling molecules in diverse fungal species with their stress resistance. These fungi, which include ascomycetes, basidiomycetes and microsporidia, occupy highly divergent niches from saline environments to plant or mammalian hosts. The fungi displayed significant variation in their resistance to osmotic (NaCl and sorbitol), oxidative (H2O2 and menadione) and cell wall stresses (Calcofluor White and Congo Red). There was no strict correlation between fungal phylogeny and stress resistance. Rather, the human pathogens tended to be more resistant to all three types of stress, an exception being the sensitivity of Candida albicans to the cell wall stress, Calcofluor White. In contrast, the plant pathogens were relatively sensitive to oxidative stress. The degree of conservation of osmotic, oxidative and cell wall stress signalling pathways amongst the eighteen fungal species was examined. Putative orthologues of functionally defined signalling components in Saccharomyces cerevisiae were identified by performing reciprocal BLASTP searches, and the percent amino acid identities of these orthologues recorded. This revealed that in general, central components of the osmotic, oxidative and cell wall stress signalling pathways are relatively well conserved, whereas the sensors lying upstream and transcriptional regulators lying downstream of these modules have diverged significantly. There was no obvious correlation between the degree of conservation of stress signalling pathways and the resistance of a particular fungus to the corresponding stress. Our data are consistent with the hypothesis that fungal stress signalling components have undergone rapid recent evolution to tune the stress responses in a niche-specific fashion.
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DOI：
10.1073/pnas.82.15.5060
发表时间：
1985-01-01
影响因子：
11.1
作者：
CASPERSON, GF;WALKER, N;BOURNE, HR
通讯作者：
BOURNE, HR
影响因子：
64.8
作者：
Dujon, B;Sherman, D;Souciet, JL
通讯作者：
Souciet, JL
影响因子：
--
作者：
Chauhan, N;Inglis, D;Calderone, R
通讯作者：
Calderone, R
影响因子：
4.2
作者：
Ashby, SF;Nowell, W
通讯作者：
Nowell, W
影响因子：
2.8
作者：
de Nobel, H;Ruiz, C;Klis, FM
通讯作者：
Klis, FM