Neural activity affects distribution of glutamate receptors during neuromuscular junction formation in Drosophila embryos.
Neural activity affects distribution of glutamate receptors during neuromuscular junction formation in Drosophila embryos.
复制标题
神经活动影响果蝇胚胎神经肌肉接头形成过程中谷氨酸受体的分布。
作者：
M. Saitoe;S. Tanaka;K. Takata;Y. Kidokoro
突触后细胞中递质受体的分布和密度的变化是功能性突触形成的必要步骤。我们提出了针对果蝇谷氨酸受体（DGluR-II）的抗体，并在胚胎神经肌肉接头形成过程中观察受体的分布。在野生型胚胎中，胚胎发育在产蛋后（AEL）22小时内完成，神经肌肉接头（NMJ）在13小时AEL开始形成。在最初的突触形成时，DGluR-IIs出现为与一些肌肉核紧密相关的簇。随后，这些nonjunctional集群分散，而DGluR-IIs积累在交界区。在麻痹性温度敏感突变体帕拉（ts 1）中，神经活动在限制性温度下急剧下降。当神经活动被阻止在整个突触形成饲养胚胎在限制性的温度之前，突触形成的开始，12小时AEL，extrajunctional集群的分散显着抑制，并没有积累的受体在交界处观察到在22小时AEL。然而，当神经活动被阻止后，饲养胚胎在限制性温度从13小时AEL，DGluR-IIs没有积累在NMJ，虽然extrajunctional集群分散正常。这些结果表明，神经活动差异调节耗散的受体簇在非交界区和受体的交界区的积累。
Changes in the distribution and density of transmitter receptors in the postsynaptic cell are required steps for functional synapse formation. We raised antibodies against Drosophila glutamate receptors (DGluR-II) and visualized the distribution of receptors during neuromuscular junction formation in embryos. In wild-type embryos, embryonic development is complete within 22 hr after egg lying (AEL) and neuromuscular junction (NMJ) formation begins at 13 hr AEL. At the time of initial synapse formation, DGluR-IIs appeared as clusters closely associated with some muscle nuclei. Subsequently, these nonjunctional clusters dispersed while DGluR-IIs accumulated at the junctional region. In a paralytic temperature-sensitive mutant, para(ts1), neural activity decreases drastically at restrictive temperatures. When neural activity was blocked throughout synaptogenesis by rearing embryos at a restrictive temperature prior to the beginning of synaptogenesis, 12 hr AEL, the dispersal of extrajunctional clusters was significantly suppressed and no accumulation of receptors at the junction was observed at 22 hr AEL. However, when neural activity was blocked later, by rearing embryos at a restrictive temperature from 13 hr AEL, DGluR-IIs did not accumulate at the NMJ, although extrajunctional clusters dispersed normally. These findings suggest that the neural activity differentially regulates dissipation of receptor clusters in the nonjunctional region and accumulation of receptors at the junctional region.
基底层成分集中在鸡胚后肢肌肉的前肌块和早期乙酰胆碱受体簇中。
影响因子：
2.7
作者：
Godfrey,EW;Siebenlist,RE;Wallskog,PA;Walters,LM;Bolender,DL;Yorde,DE
通讯作者：
Yorde,DE