Establishment of repeated liver biopsy technique in experimental mice.
Establishment of repeated liver biopsy technique in experimental mice.
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DOI：
10.1016/j.heliyon.2023.e16978
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发表时间：
2023-06
期刊：
影响因子：
4
通讯作者：
Tsuneyama, Koichi
中科院分区：
文献类型：
--
作者：
Shao, Wenhua;Ichimura-Shimizu, Mayuko;Ogawa, Hirohisa;Jin, Shengjian;Sutoh, Mitsuko;Nakamura, Satoko;Onodera, Miki;Tawara, Hirosuke;Toyohara, Shunji;Hokao, Ryoji;Kudo, Yasusei;Oya, Takeshi;Tsuneyama, Koichi
活检是一种常用的直接利用人体组织和细胞进行病理诊断的方法。活组织检查被广泛用于确定疾病进展和治疗效果。虽然器官和组织通常是在动物实验中通过牺牲获得的，但从理论上讲，在人类身上使用同样的活检技术是可能的。在本研究中，我们研究了在自发性代谢综合征小鼠模型中进行四次重复肝活检的可行性。尽管有一小部分小鼠意外死亡，但大多数小鼠都能够接受四次肝脏活检，没有发生重大不良事件。我们还在4、8和12周龄的小鼠肝癌致癌模型上进行了三次肝脏活检。除了在16周龄时采集的样本外，我们还成功地从同一小鼠的不同疾病发展阶段收集了四个肝脏样本。这种肝脏活检技术的应用可能使随着时间的推移直接评估同一个体的病理状况成为可能，从而减少实验动物的数量。
Biopsy is a commonly used method for determining pathological diagnoses by directly using human tissues and cells. Biopsies are widely used to determine disease progression and treatment efficacy. Although organs and tissues are usually obtained by sacrifice during animal experiments, it is theoretically possible to use the same biopsy techniques in humans. In the present study, we examined the feasibility of performing four repeated liver biopsies in a spontaneous metabolic syndrome mouse model. Even though a small number of mice died accidently, most mice were able to undergo four liver biopsies without significant adverse events. We also performed three liver biopsies in mouse liver tumor carcinogen models at 4, 8, and 12 weeks of age. In addition to the sample collected at 16 weeks of age during sacrifice, we successfully collected four liver samples from the same mice at different stages of disease progression. The application of this liver biopsy technique might make it possible for direct evaluation of pathological conditions in the same individual over time, thereby reducing the number of experimental animals.
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影响因子：
3.7
作者：
Shapira, Yami;Katz, Meirav;Elinav, Eran
通讯作者：
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DOI：
10.1016/j.jcmgh.2022.09.013
发表时间：
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作者：
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