The influence of three-dimensional nanofibrous scaffolds on the osteogenic differentiation of embryonic stem cells.
The influence of three-dimensional nanofibrous scaffolds on the osteogenic differentiation of embryonic stem cells.
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DOI：
10.1016/j.biomaterials.2009.01.009
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发表时间：
2009-05
期刊：
影响因子：
14
通讯作者：
Ma, Peter X.
中科院分区：
文献类型：
--
作者：
Smith, Laura A.;Liu, Xiaohua;Hu, Jiang;Ma, Peter X.
胚胎干细胞为组织工程应用提供了一种潜在的无限细胞来源。然而，为了用于这类应用，胚胎干细胞的分化必须控制在所需的谱系上。在这项研究中，我们在体外检测了纳米纤维结构和生化线索对胚胎干细胞成骨分化的影响，与更传统的结构相比，在二维(薄基质或平板薄膜)和三维(支架)中没有纳米纤维特征。培养3周后，纳米纤维基质能够支持胚胎干细胞成骨分化标记物的mRNA表达，而固体膜需要成骨补充剂和生长因子才能实现成骨分化标记物的mRNA表达。与实壁支架、纳米纤维薄基质或固体薄膜相比，纳米纤维支架显著增强成骨分化标志物的mRNA表达。培养4周后，发现纳米纤维支架的钙含量是实壁支架的3倍，骨钙素染色更强。总体而言，在二维和三维培养中，与实壁结构相比，纳米纤维结构增强了胚胎干细胞的成骨分化和矿化。
Embryonic stem cells represent a potentially unlimited cell source for tissue engineering applications. However, in order to be used for such applications, embryonic stem cells differentiation must be controlled to only the desired lineages. In this study, we examine the effects of nanofibrous architecture and biochemical cues on the osteogenic differentiation of embryonic stem cells compared to the more traditional architecture without the nanofibrous features in two dimensions (thin matrix or flat films) and three dimensions (scaffolds) in vitro. After three weeks of culture the nanofibrous thin matrices were capable of supporting mRNA expression of osteogenic differentiation markers in embryonic stem cells without osteogenic supplements, while solid films required osteogenic supplements and growth factors to achieve mRNA expression of osteogenic differentiation markers. Nanofibrous scaffolds substantially enhanced mRNA expression of osteogenic differentiation markers compared to solid-walled scaffolds, nanofibrous thin matrices or solid films. After 4 weeks of culture, nanofibrous scaffolds were found to contain 3 times more calcium and stronger osteocalcin stain throughout the scaffolds than the solid-walled scaffolds. Overall, the nanofibrous architecture enhanced the osteogenic differentiation and mineralization of embryonic stem cells compared to the solid-walled architecture in both two and three dimensional cultures.
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