Chromosome Aberrations Determined by FISH in Radiation Workers from the Sellafield Nuclear Facility
Chromosome Aberrations Determined by FISH in Radiation Workers from the Sellafield Nuclear Facility
复制标题
通过 FISH 测定塞拉菲尔德核设施辐射工作人员的染色体畸变
作者：
E. Tawn;G. B. Curwen;P. Jonas;M. Gillies;L. Hodgson;K. Cadwell
使用荧光原位杂交（FISH）检查了职业暴露于外部电离辐射源的塞拉菲尔德核设施（英国坎布里亚郡）的工人外周血淋巴细胞的易位频率。这是早期对退休工人研究的延伸，包括对早期收集的额外样本的分析，使总数达到321。另外164个样本来自现任和退休员工，包括26个重复样本，从一个新的集合中获得，从而得到一个459名工人的组合数据集。这一全部为男性的工人群体被分为6个剂量组，其中97人记录的外部职业剂量<50 mGy，118人为50-249 mGy，129人为250-499 mGy，89人为500-749 mGy，17人为750-999 mGy，9人> 1 000 mGy。单变量分析显示，外照射剂量与易位频率之间存在显著相关性（P < 0.001），斜率±标准误估计值为1.174 ± 0.164 × 10−2个易位/戈伊。多因素分析显示，年龄使易位率每年增加0.0229 ± 0.0052 × 10−2（P < 0.001）。然而，年龄调整对易位频率辐射剂量反应的影响较小，斜率±标准误差的新估计值为1.163 ± 0.162 × 10−2个易位/戈伊。随着体内慢性低LET辐射诱导易位的剂量反应现已得到很好的表征，细胞遗传学分析可在受照人群流行病学研究中对慢性照射的回顾性剂量重建中发挥不可或缺的作用。
Workers from the Sellafield nuclear facility (Cumbria, UK) with occupational exposures to external sources of ionizing radiation were examined for translocation frequencies in peripheral blood lymphocytes using fluorescence in situ hybridization (FISH). This is an extension of an earlier study of retired workers, and includes analyses of additional samples from the earlier collection, bringing the total to 321. Another 164 samples from both current and retired employees, including 26 repeat samples, were obtained from a new collection, thus giving a combined dataset of 459 workers. This all-male population of workers was divided into 6 dose groups comprising 97 with recorded external occupational doses <50 mGy, 118 with 50–249 mGy, 129 with 250–499 mGy, 89 with 500–749 mGy, 17 with 750–999 mGy and 9 with >1,000 mGy. Univariate analysis showed a significant association between external dose and translocation frequency (P < 0.001) with the estimate of slope ± standard error being 1.174 ± 0.164 × 10−2 translocations per Gy. Multivariate analysis revealed that age increased the rate of translocations by 0.0229 ± 0.0052 × 10−2 per year (P < 0.001). However, the impact of age adjustment on the radiation dose response for translocation frequencies was minor with the new estimate of slope ± standard error being 1.163 ± 0.162 × 10−2 translocations per Gy. With the dose response for the induction of translocations by chronic in vivo low-LET radiation now well characterized, cytogenetic analysis can play an integral role in retrospective dose reconstruction of chronic exposure in epidemiological studies of exposed populations.
对塞拉菲尔德核设施中职业暴露于辐射的工人进行染色体分析。
影响因子：
2.6
作者：
Tawn,EJ;Whitehouse,CA;Holdsworth,D;Morris,S;Tarone,RE
通讯作者：
Tarone,RE