Fibroblast growth factor inhibits epidermal growth factor-induced responses in rat astrocytes.
Fibroblast growth factor inhibits epidermal growth factor-induced responses in rat astrocytes.
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成纤维细胞生长因子抑制大鼠星形胶质细胞中表皮生长因子诱导的反应。
作者：
Huff,KR;Schreier,W
调节星形胶质细胞增殖的信号与正常发育过程、生长控制的转化性丧失以及许多大脑疾病状态下的反应性胶质增生有关。在原代培养的大鼠星形胶质细胞中，我们研究了两种生长因子--表皮生长因子(EGF)和成纤维细胞生长因子(FGF)--的顺序相互作用，这可能与这些条件下的大脑有关。EGF是一种强烈的有丝分裂原和2-脱氧葡萄糖(2-DG)转运的刺激剂，对细胞的培养没有影响，而成纤维细胞生长因子是一种较少的有丝分裂原和2-DG摄取刺激因子。然而，当细胞给予成纤维细胞生长因子作为预处理时，成纤维细胞生长因子强烈抑制EGF刺激DNA合成和2-DG摄取的能力。成纤维细胞生长因子对EGF刺激的抑制作用跨越EGF剂量-反应曲线，在高、低培养密度下均存在，且至少稳定3天。与EGF诱导的刺激相比，PDGF对胎牛血清诱导的刺激的抑制作用要小得多，这表明了PDGF的特异性。细胞计数证实，成纤维细胞生长因子预处理也抑制了EGF对细胞分裂的刺激作用。由于成纤维细胞生长因子的脑来源、血管生成和神经营养功能，它可能在发育、胶质瘤转化和神经再生等过程中调节EGF-星形胶质细胞的相互作用。
The signals which regulate the proliferation of astrocytes have relevance to normal developmental processes, transformational loss of growth control, and reactive gliosis present in many brain disease states. We have studied, in primary cultures of rat astrocytes, a sequential interaction of two growth factors, epidermal growth factor (EGF) and fibroblast growth factor (FGF), which may be relevant to the brain in these conditions. EGF is a strong mitogen and stimulator of 2 deoxyglucose (2 DG) transport with no effect on plating of cells, and FGF is a lesser mitogen and 2 DG uptake stimulator. However, when FGF is given to the cells as a pretreatment, FGF strongly inhibits the ability of EGF to stimulate both DNA synthesis and 2 DG uptake. The inhibition of EGF stimulation by FGF is across the EGF dose‐response curve, present at high and low culture densities, and stable for at least 3 days. Specificity is indicated by lack of inhibition by PDGF pretreatment and much less inhibition of fetal calf serum‐induced stimulations than EGF‐induced stimulation. Cell counts confirmed that the FGF pretreatment also inhibits EGF stimulation of cell division. Because of FGF brain derivation and angiogenic and neurotropic functions, it may serve as a regulator of EGF‐astrocyte interactions in processes such as development, gliomatous transformation, and neural regeneration.
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炎症性肠病患者血清血管内皮生长因子水平升高。
影响因子：
1.9
作者：
Th. Griga;A. Winse;A. Tromm;B. May
通讯作者：
B. May
Flt-1 是血管内皮生长因子的受体，具有转化和形态发生潜力
影响因子：
8
作者：
Y. Maru;S. Yamaguchi;M. Shibuya
通讯作者：
M. Shibuya
DOI：
10.1165/ajrcmb.17.1.2796
发表时间：
1997-07-01
影响因子：
6.4
作者：
Horiuchi, T;Weller, PF
通讯作者：
Weller, PF
DOI：
10.1073/pnas.93.10.4851
发表时间：
1996-05-14
影响因子：
11.1
作者：
Robinson, GS;Pierce, EA;Smith, LEH
通讯作者：
Smith, LEH
影响因子：
8
作者：
M. Shibuya;S. Yamaguchi;A. Yamane;T. Ikeda;A. Tojo;H. Matsushime;Misako Sato
通讯作者：
M. Shibuya;S. Yamaguchi;A. Yamane;T. Ikeda;A. Tojo;H. Matsushime;Misako Sato