Quantitating the subtleties of microglial morphology with fractal analysis.
Quantitating the subtleties of microglial morphology with fractal analysis.
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DOI：
10.3389/fncel.2013.00003
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发表时间：
2013
影响因子：
5.3
通讯作者：
Jelinek HF
中科院分区：
文献类型：
--
作者：
Karperien A;Ahammer H;Jelinek HF
众所周知，小胶质细胞的形态和功能是密不可分的。近年来，通过先进的成像技术，即使在目前公认的形态学范畴内，小胶质细胞形态也是高度动态的，这一传统观点得到了强调，即小胶质细胞形态介于分支静息状态和激活的阿米巴样体之间。此外，小胶质细胞在不同的类别之间采用有意义的中间形式，在功能上有相当大的交叉，在它们循环、迁移、波动、吞噬以及延伸和收缩细小和粗大过程时，形态也不同。从定量的角度来看，用传统方法衡量这种变异性是有问题的，但量化这种细节的一种方法是通过分形分析。分形分析技术已被用于对小胶质细胞的形态进行量化，对粗略差异进行分类，但也用于区分细微差异(例如，在分支细胞之间)。尤其是多重分形分析是一种可能对识别中间形式有用的分形分析技术。在这里，我们回顾了当前分形分析的趋势和方法，重点是盒计数分析，包括空隙分析和多重分形分析，应用于小胶质细胞形态。
It is well established that microglial form and function are inextricably linked. In recent years, the traditional view that microglial form ranges between “ramified resting” and “activated amoeboid” has been emphasized through advancing imaging techniques that point to microglial form being highly dynamic even within the currently accepted morphological categories. Moreover, microglia adopt meaningful intermediate forms between categories, with considerable crossover in function and varying morphologies as they cycle, migrate, wave, phagocytose, and extend and retract fine and gross processes. From a quantitative perspective, it is problematic to measure such variability using traditional methods, but one way of quantitating such detail is through fractal analysis. The techniques of fractal analysis have been used for quantitating microglial morphology, to categorize gross differences but also to differentiate subtle differences (e.g., amongst ramified cells). Multifractal analysis in particular is one technique of fractal analysis that may be useful for identifying intermediate forms. Here we review current trends and methods of fractal analysis, focusing on box counting analysis, including lacunarity and multifractal analysis, as applied to microglial morphology.
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