Application of Advanced Light Microscopy to the Study of HIV and Its Interactions with the Host.

Application of Advanced Light Microscopy to the Study of HIV and Its Interactions with the Host.
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DOI:
10.3390/v13020223
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发表时间:
2021-02-01
期刊:
Viruses
影响因子:
--
通讯作者:
Saffarian S
Saffarian S
中科院分区:
其他
文献类型:
--
作者:
Saffarian S

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这篇综述强调了人类免疫缺陷病毒(HIV)的组装,释放和成熟的先进光学显微镜技术的重要观察。现在,使这些光学显微镜测量技术的进步进行了讨论,特别强调现场成像方法,包括全内反射荧光(TIRF),高分辨率光学显微镜技术,包括PALM和STORM和单分子测量,包括荧光共振能量转移(FRET)。审查的结论与讨论什么新的见解和理解,可以预期从这些测量。
This review highlights the significant observations of human immunodeficiency virus (HIV) assembly, release and maturation made possible with advanced light microscopy techniques. The advances in technology which now enables these light microscopy measurements are discussed with special emphasis on live imaging approaches including Total Internal Reflection Fluorescence (TIRF), high-resolution light microscopy techniques including PALM and STORM and single molecule measurements, including Fluorescence Resonance Energy Transfer (FRET). The review concludes with a discussion on what new insights and understanding can be expected from these measurements.
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