Macrophage colony-stimulating factor (rM-CSF) stimulates pinocytosis in bone marrow-derived macrophages.
Macrophage colony-stimulating factor (rM-CSF) stimulates pinocytosis in bone marrow-derived macrophages.
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DOI：
10.1084/jem.170.5.1635
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发表时间：
1989-11-01
影响因子：
15.3
通讯作者：
SWANSON, JA
中科院分区：
文献类型：
--
作者：
RACOOSIN, EL;SWANSON, JA
在含有重组巨噬细胞集落刺激因子（rM-CSF）的培养基中孵育小鼠骨髓源性巨噬细胞（BMM）可刺激液相胞饮标记物荧光黄（LY）的流入、流出和净蓄积。刺激是剂量依赖性的，在添加生长因子后5分钟内发生，并且持续。以前的实验表明，PMA处理的BMM被刺激积累LY，但与rM-CSF处理的细胞相比，PMA处理的巨噬细胞中的刺激的开始较慢。在rM-CSF和PMA刺激的LY积累的进一步比较中，发现rM-CSF刺激的胞饮作用可以通过用0.5 mg/ml胰蛋白酶预处理而消除，而胰蛋白酶预处理既不影响未刺激的LY积累，也不影响PMA刺激的LY积累。这些发现表明，rM-CSF反应是在细胞表面启动的，而PMA反应是通过细胞内（或胰蛋白酶抗性）受体发生的。然而，一旦启动，由任一药剂引起的胞饮反应非常相似。首先，rM-CSF处理的细胞，像PMA处理的细胞，表现出广泛的皱褶和大相亮胞饮体的形成。第二，rM-CSF和PMA刺激的LY积累可以通过用细胞骨架去稳定化药物诺考达唑、秋水仙碱或细胞松弛素D处理细胞来抑制。最后，发现rM-CSF，像PMA一样，刺激LY从预先装载染料的细胞中流出。因此，rM-CSF和PMA均刺激溶质流过巨噬细胞内吞隔室的净速率。
Incubation of murine bone marrow-derived macrophages (BMM) in medium containing recombinant macrophage colony-stimulating factor (rM-CSF) stimulated influx, efflux, and the net accumulation of the fluid-phase pinocytic marker, lucifer yellow (LY). Stimulation was dose dependent, occurred within 5 min of addition of the growth factor, and was sustained. Previous experiments had shown that BMM treated with PMA were stimulated to accumulate LY, but compared with rM-CSF-treated cells, the onset of stimulation in PMA-treated macrophages was slower. In further comparisons of rM-CSF- and PMA-stimulated LY accumulation, it was found that rM-CSF-stimulated pinocytosis could be abolished by pretreatment with 0.5 mg/ml trypsin, whereas neither unstimulated nor PMA-stimulated LY accumulation was affected by trypsin pretreatment. These findings indicate that the rM-CSF response was initiated at the cell surface, while the PMA response occurred via intracellular (or trypsin-resistant) receptors. However, once initiated, the pinocytic responses elicited by either agent were very similar. First, rM-CSF- treated cells, like PMA-treated cells, showed extensive ruffling and formation of large phase-bright pinosomes. Second, both rM-CSF- and PMA- stimulated LY accumulation could be inhibited by treatment of cells with the cytoskeleton destabilizing drugs nocodazole, colchicine, or cytochalasin D. Finally, rM-CSF, like PMA, was found to stimulate efflux of LY from cells preloaded with the dye. Thus, both rM-CSF and PMA stimulate the net rate of solute flow through the macrophage endocytic compartment.
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影响因子：
3.7
作者：
MIYATA, Y;HOSHI, M;SAKAI, H
通讯作者：
SAKAI, H
影响因子：
7.8
作者：
SWANSON, JA;YIRINEC, BD;SILVERSTEIN, SC
通讯作者：
SILVERSTEIN, SC
影响因子：
7.8
作者：
TANNER, LI;LIENHARD, GE
通讯作者：
LIENHARD, GE
影响因子：
64.8
作者：
TSIEN, RY;POZZAN, T;RINK, TJ
通讯作者：
RINK, TJ
影响因子：
64.5
作者：
TUSHINSKI, RJ;OLIVER, IT;STANLEY, ER
通讯作者：
STANLEY, ER