A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. II. Effects on cell movement, organization of microtubules, and intermediate filaments, and arrangement of Golgi elements
A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. II. Effects on cell movement, organization of microtubules, and intermediate filaments, and arrangement of Golgi elements
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一种与酪氨酸化形式的 α-微管蛋白发生特异性反应的大鼠单克隆抗体。
DOI：
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发表时间：
1983
影响因子：
7.8
通讯作者：
M. Willingham
中科院分区：
文献类型：
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作者：
J. Wehland;M. Willingham
大鼠抗酵母α -微管蛋白单克隆抗体（克隆YL 1/2）； Kilmartin， J. V. B. Wright， C. Milstein, 1982，细胞生物学杂志。（见Wehland， J.， M. C. Willingham, and I. V. Sandoval, 1983， J. Cell Biol.）。微管在活细胞中的组织和功能。根据YL 1/2的注射浓度不同，观察到以下惊人的效果：(a)注射低浓度时（注射溶液中2mg IgG/ml），微管被装饰而不改变其分布，细胞器的胞内运动（跳跃运动）和细胞移位不受影响。中等浓度（6 mg IgG/ml）诱导微管成束但不发生核周聚集，可消除跳跃运动和细胞易位，高浓度（大于12 mg IgG/ml）诱导微管核周聚集也有同样的效果。(b) YL 1/2在中、高浓度注射时，阻滞细胞有丝分裂。这些细胞没有正常的纺锤体结构。(c)注射中间浓度的YL 1/2阻止跳跃运动，导致中间细丝很少或没有聚集，也没有高尔基复合体的分散。注射高浓度后，导致微管在核周聚集，中间细丝形成核周束，高尔基复合体变得分散，类似于用秋碱处理细胞后的结果。(d)若罗丹明偶联YL 1/2以停止跳跃运动和阻止细胞有丝分裂的浓度注射，可通过视频图像增强显微镜观察活细胞的微管结构并跟踪数小时。在观察过程中，它们的分布和组织几乎没有变化，尽管这些微管结构似乎没有被注射YL 1/2稳定，因为它们分别容易被colcolid或冷处理解聚，并在药物去除或重新加热到37℃时重新聚合。这些结果在微管参与细胞活动（如细胞运动和细胞质组织）和YL 1/2对酪氨酸化形式的α -微管蛋白的特异性方面进行了讨论。
A rat monoclonal antibody against yeast alpha-tubulin (clone YL 1/2; Kilmartin, J. V., B. Wright, and C. Milstein, 1982, J. Cell Biol., 93:576-582) that reacts specifically with the tyrosylated form of alpha- tubulin and readily binds to tubulin in microtubules when injected into cultured cells (see Wehland, J., M. C. Willingham, and I. V. Sandoval, 1983, J. Cell Biol., 97:1467-1475) was used to study microtubule organization and function in living cells. Depending on the concentration of YL 1/2 that was injected the following striking effects were observed: (a) When injected at a low concentration (2 mg IgG/ml in the injection solution), where microtubules were decorated without changing their distribution, intracellular movement of cell organelles (saltatory movement) and cell translocation were not affected. Intermediate concentrations (6 mg IgG/ml) that induced bundling but no perinuclear aggregation of microtubules abolished saltatory movement and cell translocation, and high concentrations (greater than 12 mg IgG/ml) that induced perinuclear aggregation of microtubules showed the same effect. (b) YL 1/2, when injected at intermediate and high concentrations, arrested cells in mitosis. Such cells showed no normal spindle structures. (c) Injection of an intermediate concentration of YL 1/2 that stopped saltatory movement caused little or no aggregation of intermediate filaments and no dispersion of the Golgi complex. After injection of high concentrations, resulting in perinuclear aggregation of microtubules, intermediate filaments formed perinuclear bundles and the Golgi complex became dispersed analogous to results obtained after treatment of cells with colcemid. (d) When rhodamine-conjugated YL 1/2 was injected at concentrations that stopped saltatory movement and arrested cells in mitosis, microtubule structures could be visualized and followed for several hours in living cells by video image intensification microscopy. They showed little or no change in distribution and organization during observation, even though these microtubule structures appeared not to be stabilized by injected YL 1/2 since they were readily depolymerized by colcemid or cold treatment and repolymerized upon drug removal or rewarming to 37 degrees C, respectively. These results are discussed in terms of the participation of microtubules in cellular activities such as cell movement and cytoplasmic organization and in terms of the specificity of YL 1/2 for the tyrosylated form of alpha-tubulin.
影响因子：
56.9
作者：
ALLEN, RD;METUZALS, J;GILBERT, SP
通讯作者：
GILBERT, SP