Sub3 Inhibits Mycelia Growth and Aflatoxin Production of Aspergillus Flavus
Sub3 Inhibits Mycelia Growth and Aflatoxin Production of Aspergillus Flavus
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Sub3 抑制黄曲霉菌丝体生长和黄曲霉毒素产生
DOI：
10.1007/s11483-021-09715-6
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发表时间：
2022-02
期刊：
影响因子：
3
通讯作者：
Yuansen Hu
中科院分区：
文献类型：
--
作者：
Wei Zhang;Yangyong Lv;Haojie Yang;Shan Wei;Shuaibing Zhang;Na Li;Yuansen Hu
Sub3, a short cationic antimicrobial peptide, exhibits potent antifungal activity against Aspergillus flavus . A model of the mechanisms through which Sub3 affects A. flavus spores has been proposed in our previous work. However, the antifungal effects of Sub3 on mycelial growth and aflatoxin production of A. flavus remain unclear. In our study, we found that Sub3 strongly inhibited mycelial growth, sporulation, and sclerotia formation on potato dextrose agar and yeast extract peptone dextrose medium at 400 μg/mL. Furthermore, production of aflatoxin B1 was reduced by 18.93% and 98.92% after treatment with 200 and 400 μg/mL Sub3, respectively. Shrivelled hyphae were observed by scanning electron microscopy following 12 h Sub3 treatment. Additionally, Sub3 destroyed membrane integrity, decreased mitochondrial membrane potential, and caused excessive nuclear condensation, as illustrated by propidium iodide, 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide and 4′,6-diamidino-2-phenylindole staining. After treatment with 100 and 150 μg/mL Sub3, intracellular malate dehydrogenase activity decreased by 46.13% and 56.58%, and succinate dehydrogenase activity decreased by 35.35% and 81.25%, respectively. Meanwhile, the pathogenicity assay demonstrated that Sub3 possessed potent antifungal activity in reducing A. flavus infection in maize seeds, as it could reduce conidia and aflatoxin production. Therefore, our findings indicate that Sub3 has significant potential as an eco-friendly antifungal agent against A. flavus in the food.
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影响因子：
3.9
作者：
Belasli A;Ben Miri Y;Aboudaou M;Aït Ouahioune L;Montañes L;Ariño A;Djenane D
通讯作者：
Djenane D
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影响因子：
5
作者：
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Yuansen Hu
影响因子：
4.1
作者：
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DOI：
10.1016/j.biocel.2017.02.006
发表时间：
2017-04
期刊：
The international journal of biochemistry & cell biology
影响因子：
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通讯作者：
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DOI：
10.1016/j.bbagen.2011.08.011
发表时间：
2011-12-01
影响因子：
3
作者：
Cho, Jaeyong;Lee, Dong Gun
通讯作者：
Lee, Dong Gun