Molecular detection of protists associated with the Manila clam Ruditapes philippinarum inhabiting the Wajiro tidal flat in Hakata Bay, Fukuoka Prefecture

Molecular detection of protists associated with the Manila clam Ruditapes philippinarum inhabiting the Wajiro tidal flat in Hakata Bay, Fukuoka Prefecture
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福冈县博多湾轮代郎滩涂马尼拉蛤菲律宾蛤仔相关原生生物的分子检测

DOI:
10.1007/s12562-020-01435-z
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发表时间:
2020
期刊:
影响因子:
1.9
通讯作者:
Otsubo M
Otsubo M
中科院分区:
农林科学4区
文献类型:
--
作者:
Takishita K;Kawai R;Tsutsumi A;Tanifuji G;Otsubo M

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潮滩在生态上是重要的,因为它们支持大型动物群落(例如,螃蟹、软体动物和多毛类动物),并恢复水质。然而,关于微生物真核生物,其中大多数是原生生物,与动物栖息在这些环境中的信息是有限的。在本研究中,利用聚合酶链反应技术,我们检测到的小亚基核糖体RNA(SSU rRNA)基因片段的原生生物的鳃组织的马尼拉蛤蛤菲律宾蛤仔在轮次郎潮滩,博多湾,日本。根据SSU rRNA基因的同源性分析,所检测到的微生物不仅属于帕金索动物门,而且还属于纤毛门、动质体门和单孢子虫门。对于隶属于Perkinsozoa的原生生物,从相同的鳃组织样品中提取rRNA基因的内转录间隔区(ITS),以在种水平上进行精确鉴定。获得的ITS序列与在日本马尼拉蛤种群中高度流行的奥氏帕金斯完全相同。我们的研究结果表明,原生生物可能寄生在这一代表性的软体动物在潮滩的生物遗传多样性,检测到的生物可能会影响宿主蛤的种群动态。
Tidal flats are ecologically important as they support a large community of animals (e.g., crabs, mollusks, and polychaetes) and restore water quality. However, information regarding microbial eukaryotes, most of which are protists, associated with animals inhabiting these environments is limited. In the present study, utilizing polymerase chain reaction techniques, we detected fragments of the small subunit ribosomal RNA (SSU rRNA) gene of protists from the gill tissues of the Manila clamRuditapes philippinarumin the Wajiro tidal flat, Hakata Bay, Japan. Based on the phylogeny of the SSU rRNA gene, the detected organisms were shown to belong to not only Perkinsozoa but also to Ciliophora, Kinetoplastida, and Haplosporidia. For the protist affiliated with Perkinsozoa, the internal transcribed spacer (ITS) of the rRNA genes was retrieved from the same gill tissue samples for precise identification at the species level. The ITS sequence obtained was identical to that ofPerkinsus olseni, which is highly prevalent in Manila clam populations in Japan. Our findings suggest that protists potentially parasitic on this representative mollusk in tidal flats are phylogenetically diverse, and that the organisms detected may affect the population dynamics of the host clams.
低盐度和低温对马尼拉蛤体内原生动物奥尔森氏珀金虫(Perkinsus olseni)繁殖的抑制作用
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