Comprehensive transcriptome analysis and flavonoid profiling of Ginkgo leaves reveals flavonoid content alterations in day-night cycles.
Comprehensive transcriptome analysis and flavonoid profiling of Ginkgo leaves reveals flavonoid content alterations in day-night cycles.
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银杏叶的综合转录组分析和类黄酮分析揭示了昼夜周期中类黄酮含量的变化
DOI：
10.1371/journal.pone.0193897
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Xu M
中科院分区：
文献类型：
--
作者：
Ni J;Dong L;Jiang Z;Yang X;Chen Z;Wu Y;Xu M
银杏叶是提取黄酮类化合物的原料。因此，其收获的时机对于优化提取效率是重要的，这有利于制药工业。在这项研究中，我们比较了银杏叶的转录组在中午和午夜收获。在类黄酮生物合成的每个步骤中具有最高概率的差异表达基因在午夜下调。此外，实时PCR证实了转录组结果，表明类黄酮生物合成在午夜减少。比较了中午和午夜采收的银杏叶中黄酮类化合物的含量，发现午夜采收的银杏叶中总黄酮含量降低。对单个黄酮类化合物的详细分析表明，大部分黄酮类化合物的含量都有不同程度的下降。研究结果表明，银杏叶中黄酮类化合物的含量受昼夜节律的影响，这为优化银杏叶的采收期提供了有价值的信息。
Ginkgo leaves are raw materials for flavonoid extraction. Thus, the timing of their harvest is important to optimize the extraction efficiency, which benefits the pharmaceutical industry. In this research, we compared the transcriptomes of Ginkgo leaves harvested at midday and midnight. The differentially expressed genes with the highest probabilities in each step of flavonoid biosynthesis were down-regulated at midnight. Furthermore, real-time PCR corroborated the transcriptome results, indicating the decrease in flavonoid biosynthesis at midnight. The flavonoid profiles of Ginkgo leaves harvested at midday and midnight were compared, and the total flavonoid content decreased at midnight. A detailed analysis of individual flavonoids showed that most of their contents were decreased by various degrees. Our results indicated that circadian rhythms affected the flavonoid contents in Ginkgo leaves, which provides valuable information for optimizing their harvesting times to benefit the pharmaceutical industry.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
6.2
作者：
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影响因子：
12.3
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Covington, Michael F.;Maloof, Julin N.;Straume, Marty;Kay, Steve A.;Harmer, Stacey L.
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氯化钠通过收获后银杏叶中假定的新途径诱导类黄酮生物合成
影响因子：
5.6
作者：
Ni J;Hao J;Jiang Z;Zhan X;Dong L;Yang X;Sun Z;Xu W;Wang Z;Xu M
通讯作者：
Xu M
影响因子：
9.2
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Chen W