High levels of reactive oxygen species in gold nanoparticle-targeted cancer cells following femtosecond pulse irradiation.
High levels of reactive oxygen species in gold nanoparticle-targeted cancer cells following femtosecond pulse irradiation.
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DOI：
10.1038/srep02146
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发表时间：
2013
影响因子：
4.6
通讯作者：
Yelin, Dvir
中科院分区：
文献类型：
--
作者：
Minai, Limor;Yeheskely-Hayon, Daniella;Yelin, Dvir
使用特定靶向的金纳米颗粒和激光脉冲照射可以局部损伤癌细胞，同时保持对附近无颗粒组织的最小损伤。在这里，我们表明，除了直接光热细胞损伤，高浓度的活性氧（ROS）的辐射细胞内形成。伯基特淋巴瘤B细胞和上皮性乳腺癌细胞被抗体包被的金纳米球靶向，并被几个共振飞秒脉冲照射，导致细胞内ROS的显著升高，其使用不同荧光标记物的延时显微镜进行表征和定量。研究结果表明，使用金纳米颗粒和超短脉冲靶向各种恶性肿瘤的技术可能比以前预期的更有效和通用，从而为局部癌症治疗提供多样化，高度特异性的工具集。
Cancer cells could be locally damaged using specifically targeted gold nanoparticles and laser pulse irradiation, while maintaining minimum damage to nearby, particle-free tissue. Here, we show that in addition to the immediate photothermal cell damage, high concentrations of reactive oxygen species (ROS) are formed within the irradiated cells. Burkitt lymphoma B cells and epithelial breast cancer cells were targeted by antibody-coated gold nanospheres and irradiated by a few resonant femtosecond pulses, resulting in significant elevation of intracellular ROS which was characterized and quantified using time-lapse microscopy of different fluorescent markers. The results suggest that techniques that involve targeting of various malignancies using gold nanoparticles and ultrashort pulses may be more effective and versatile than previously anticipated, allowing diverse, highly specific set of tools for local cancer therapy.
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DOI：
10.1073/pnas.2232479100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Hirsch, LR;Stafford, RJ;West, JL
通讯作者：
West, JL
影响因子：
2.4
作者：
Huang, Xiaohua;Qian, Wei;El-Sayed, Mostafa A.
通讯作者：
El-Sayed, Mostafa A.
影响因子：
10.8
作者：
Krpetic, Zeljka;Nativo, Paola;Volk, Martin
通讯作者：
Volk, Martin
影响因子：
3.4
作者：
Anderson, CW;Dunn, JJ;Allalunis-Turner, MJ
通讯作者：
Allalunis-Turner, MJ
DOI：
10.1364/josab.29.001383
发表时间：
2012-06-01
影响因子：
1.9
作者：
Bisker, Gili;Yelin, Dvir
通讯作者：
Yelin, Dvir