Osteoprotegerin in exosome-like vesicles from human cultured tubular cells and urine.
Osteoprotegerin in exosome-like vesicles from human cultured tubular cells and urine.
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DOI：
10.1371/journal.pone.0072387
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ortiz A
中科院分区：
文献类型：
--
作者：
Benito-Martin A;Ucero AC;Zubiri I;Posada-Ayala M;Fernandez-Fernandez B;Cannata-Ortiz P;Sanchez-Nino MD;Ruiz-Ortega M;Egido J;Alvarez-Llamas G;Ortiz A
尿外泌体已被提出作为潜在的诊断工具。TNF超家族细胞因子和受体可存在于外泌体中并由近端肾小管细胞表达。我们现在已经研究了所选TNF超家族蛋白在来自培养的人近端肾小管细胞和人尿液的外泌体样囊泡中的表达，并通过LC-MS/MS蛋白质组学鉴定了这些囊泡中的其他蛋白质。人近端肾小管细胞组成性释放不含TNF超家族细胞因子TRAIL或TWEAK的外泌体样囊泡。然而，外泌体样囊泡含有骨保护素（OPG），TNF受体超家族蛋白质，如通过Western印迹，ELISA或选择反应监测nLC-（QQQ）MS/MS评估。二十一个额外的蛋白质被确定在肾小管细胞外泌体样囊泡，包括一个（维生素D结合蛋白），以前没有报道的外泌体样囊泡。12种是细胞外基质蛋白，包括基底膜蛋白IV型胶原、巢蛋白-1、聚集蛋白和纤蛋白-1。来自慢性肾病患者的尿液比来自健康志愿者的尿液含有更高量的外泌体蛋白和外泌体OPG。具体来说，OPG在常染色体显性多囊肾病尿外泌体样囊泡中增加，并在体内由囊性上皮表达。总之，OPG存在于近端肾小管上皮细胞分泌的外泌体样囊泡中，并从慢性肾病尿液中分离。
Urinary exosomes have been proposed as potential diagnostic tools. TNF superfamily cytokines and receptors may be present in exosomes and are expressed by proximal tubular cells. We have now studied the expression of selected TNF superfamily proteins in exosome-like vesicles from cultured human proximal tubular cells and human urine and have identified additional proteins in these vesicles by LC-MS/MS proteomics. Human proximal tubular cells constitutively released exosome-like vesicles that did not contain the TNF superfamily cytokines TRAIL or TWEAK. However, exosome-like vesicles contained osteoprotegerin (OPG), a TNF receptor superfamily protein, as assessed by Western blot, ELISA or selected reaction monitoring by nLC-(QQQ)MS/MS. Twenty-one additional proteins were identified in tubular cell exosome-like vesicles, including one (vitamin D binding protein) that had not been previously reported in exosome-like vesicles. Twelve were extracellular matrix proteins, including the basement membrane proteins type IV collagen, nidogen-1, agrin and fibulin-1. Urine from chronic kidney disease patients contained a higher amount of exosomal protein and exosomal OPG than urine from healthy volunteers. Specifically OPG was increased in autosomal dominant polycystic kidney disease urinary exosome-like vesicles and expressed by cystic epithelium in vivo. In conclusion, OPG is present in exosome-like vesicles secreted by proximal tubular epithelial cells and isolated from Chronic Kidney Disease urine.
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影响因子：
14.9
作者：
Jensen LJ;Kuhn M;Stark M;Chaffron S;Creevey C;Muller J;Doerks T;Julien P;Roth A;Simonovic M;Bork P;von Mering C
通讯作者：
von Mering C
影响因子：
3.7
作者：
Miner, Jeffrey H.
通讯作者：
Miner, Jeffrey H.
影响因子：
4
作者：
Hakulinen, Juha;Sankkila, Lotta;Keski-Oja, Jorma
通讯作者：
Keski-Oja, Jorma
DOI：
10.1016/j.bbrc.2012.02.099
发表时间：
2012-03-30
影响因子：
3.1
作者：
Kulkarni, Rishikesh N.;Bakker, Astrid D.;Klein-Nulend, Jenneke
通讯作者：
Klein-Nulend, Jenneke
影响因子：
6.4
作者：
Janowska-Wieczorek, A;Wysoczynski, M;Ratajczak, MZ
通讯作者：
Ratajczak, MZ