An unusual, CD4 and CD8 dual-positive, CD25 negative, tumor cell phenotype in a patient with adult T-cell leukemia/lymphoma
An unusual, CD4 and CD8 dual-positive, CD25 negative, tumor cell phenotype in a patient with adult T-cell leukemia/lymphoma
复制标题
成人 T 细胞白血病/淋巴瘤患者中不寻常的 CD4 和 CD8 双阳性、CD25 阴性肿瘤细胞表型
DOI：
10.1080/10428194.2018.1439168
复制
发表时间：
2018
影响因子：
2.6
通讯作者：
Yasukawa Masaki
中科院分区：
文献类型：
--
作者：
Casey Nicholas;Fujiwara Hiroshi;Azuma Taichi;Murakami Yuichi;Yoshimitsu Makoto;Masamoto Izumi;Nawa Yuichiro;Yamanouchi Jun;Narumi Hiroshi;Yakushijin Yoshihiro;Hato Takaaki;Yasukawa Masaki
成人T细胞白血病/淋巴瘤（ATLL）是一种独特类型的外周T细胞肿瘤，其特征是具有多形性（花状）核的转化T淋巴细胞，并且是由于感染人T细胞白血病病毒I型（HTLV-I）所致。ATLL是区域性地方病，特别是在日本西南部，加勒比海盆地和中非部分地区。肿瘤细胞通常类似于成熟的辅助性T细胞，表达CD 3、CD 4和CD 25抗原，并且对CD 8呈阴性[1]。然而，目前还不清楚ATLL如何在宿主细胞感染后发展。在此，我们的目的是阐明这些机制，通过描述的情况下，其肿瘤细胞表达一个不寻常的CD 4 +/CD 8 +/CD 25+表型，这些细胞的表观遗传学检查。一个78岁的日本妇女被称为爱媛大学医院全身淋巴结病。入院时，全血细胞计数显示白色细胞计数为42 109/L，包括17.6%的淋巴细胞，但缺乏多形性细胞。血红蛋白为11.7 g/dL，血小板计数为231109/L。血清乳酸脱氢酶记录为570 IU/L（85-253 IU/L）。血清钙浓度未升高。血清中检测到抗HTLV-1抗体。Southern印迹随后鉴定了所有ATLL肿瘤细胞共有的单个HTLV-1整合位点，证实它们是单克隆的。通过反向聚合酶链反应（I-PCR）测序将前病毒整合位点定位于染色体位置5 q11 -12; Polo样激酶2（PLK 2）基因下游约1 kb。计算机断层扫描和镓造影显示颈部、腋窝、腹股沟、主动脉旁和肠系膜淋巴结异常肿胀。对颈部淋巴结进行活检，
Adult T-cell leukemia/lymphoma (ATLL) is a distinct type of peripheral T-cell neoplasm characterized by transformed T-lymphocytes with a pleomorphic (flower-like) nucleus and is resulted due to an infection with human T-cell leukemia virus type I (HTLV-I). ATLL is regionally endemic, notably in southwestern parts of Japan, the Caribbean basin, and parts of Central Africa. Tumor cells typically resemble mature helper T-cells, expressing CD3, CD4 and CD25 antigens, and are negative for CD8 [1]. However, it is as yet unclear how ATLL develops following infection of host cells. Herein we aim to shed light on these mechanisms, by describing the case of a patient whose tumor cells expressed an unusual CD4 þ/CD8 þ/CD25 À phenotype, with an examination of the epigenetics of these cells.A 78-year-old Japanese woman was referred to Ehime University Hospital with systemic lymphadenopathy. On admission, a complete blood count showed a white cell count of 42Â 109/L, comprising 17.6% lymphocytes, but lacking pleomorphic cells. Hemoglobin was 11.7 g/dL, and the platelet count was 231Â109/L. Serum lactate dehydrogenase was recorded at 570IU/L (85–253 IU/L). The serum calcium concentration was not elevated. Antibodies against HTLV-1 were detected in the serum. Southern blotting subsequently identified a single HTLV-1 integration site common to all ATLL tumor cells, confirming that they were monoclonal. Sequencing by inverse polymerase chain reaction (I-PCR) mapped the proviral integration site to chromosome location 5q11-12; approximately 1kb downstream of the Polo-like kinase 2 (PLK2) gene. Computed tomography scanning and gallium scintigraphy revealed abnormal swelling of cervical, axillary, inguinal, para-aortic, and mesenteric lymph nodes. A cervical lymph node was biopsied and
16 名成人 T 细胞白血病/淋巴瘤患者的白血病细胞的表型和功能分析。
影响因子：
20.3
作者：
Y. Yamada
通讯作者：
Y. Yamada
影响因子：
82.9
作者：
Hasegawa, H;Sawa, H;Hall, WW
通讯作者：
Hall, WW