Characterization and genomic analysis of kraft lignin biodegradation by the beta-proteobacterium Cupriavidus basilensis B-8.
Characterization and genomic analysis of kraft lignin biodegradation by the beta-proteobacterium Cupriavidus basilensis B-8.
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DOI：
10.1186/1754-6834-6-1
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发表时间：
2013-01-08
影响因子：
6.3
通讯作者：
Zheng Y
中科院分区：
文献类型：
--
作者：
Shi Y;Chai L;Tang C;Yang Z;Zhang H;Chen R;Chen Y;Zheng Y
木质素原料丰富，是生产生物燃料的最重要的潜在来源之一。开发一种高效的木质素降解工艺在生产包括生物乙醇在内的各种化学品方面具有相当大的潜力。然而，用现有的方法降解木质素效率很低。细菌具有极强的环境适应性和生物化学多样性，可作为快速降解木质素的有价值的工具。硫酸盐木质素(KL)是制浆造纸工业碱法硫化处理木质纤维素的副产物，广泛应用于木质素的相关研究。从腐蚀性竹简中分离到的β-变形杆菌Cupravidus basilensis B-8具有较强的KL降解能力。在初始浓度为0.5~6g L-1的条件下，7d内KL的降解率至少为31.3%。初始浓度为2g L-1时，降解率最高为44.4%。KL降解的最适pH为7.0，最适温度为30℃。锰过氧化物酶和漆酶在培养第3天和第4天活性最高，分别为1685.3 U L~(-1)和815.6 U L~(-1)。GC-MS分析表明，KL在降解过程中生成了许多小分子中间体。为了在该细菌中进行木质素降解的代谢重建，我们获得了巴氏弧菌B-8的基因组草图序列。基因组分析的重点是该细菌对几种木质素衍生化合物的分解代谢能力。这些分析结合序列比较预测了三种主要代谢途径的存在：β-酮己二酸酯途径、苯酚降解途径和龙胆酸途径。这些结果证实了巴氏梭菌B-8具有促进KL降解的能力。对巴氏梭菌B-8基因组进行全基因组测序和系统分析，确定了这种细菌介导的KL降解方法的降解步骤和中间产物。我们的研究结果为木质素降解机理的研究提供了理论基础，也为利用木质素原料生产生物燃料提供了实践依据。
Lignin materials are abundant and among the most important potential sources for biofuel production. Development of an efficient lignin degradation process has considerable potential for the production of a variety of chemicals, including bioethanol. However, lignin degradation using current methods is inefficient. Given their immense environmental adaptability and biochemical versatility, bacterial could be used as a valuable tool for the rapid degradation of lignin. Kraft lignin (KL) is a polymer by-product of the pulp and paper industry resulting from alkaline sulfide treatment of lignocellulose, and it has been widely used for lignin-related studies. Beta-proteobacterium Cupriavidus basilensis B-8 isolated from erosive bamboo slips displayed substantial KL degradation capability. With initial concentrations of 0.5–6 g L-1, at least 31.3% KL could be degraded in 7 days. The maximum degradation rate was 44.4% at the initial concentration of 2 g L-1. The optimum pH and temperature for KL degradation were 7.0 and 30°C, respectively. Manganese peroxidase (MnP) and laccase (Lac) demonstrated their greatest level of activity, 1685.3 U L-1 and 815.6 U L-1, at the third and fourth days, respectively. Many small molecule intermediates were formed during the process of KL degradation, as determined using GC-MS analysis. In order to perform metabolic reconstruction of lignin degradation in this bacterium, a draft genome sequence for C. basilensis B-8 was generated. Genomic analysis focused on the catabolic potential of this bacterium against several lignin-derived compounds. These analyses together with sequence comparisons predicted the existence of three major metabolic pathways: β-ketoadipate, phenol degradation, and gentisate pathways. These results confirmed the capability of C. basilensis B-8 to promote KL degradation. Whole genomic sequencing and systematic analysis of the C. basilensis B-8 genome identified degradation steps and intermediates from this bacterial-mediated KL degradation method. Our findings provide a theoretical basis for research into the mechanisms of lignin degradation as well as a practical basis for biofuel production using lignin materials.
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影响因子：
5
作者：
Hernandez, M;Rodriguez, J;Arias, ME
通讯作者：
Arias, ME
影响因子：
14.9
作者：
Lagesen K;Hallin P;Rødland EA;Staerfeldt HH;Rognes T;Ussery DW
通讯作者：
Ussery DW
从受侵蚀的竹简中分离出的丛毛单胞菌 B-9 菌株对硫酸盐木质素的生物降解
影响因子：
4
作者：
Chen, Y. H.;Chai, L. Y.;Zhang, H.
通讯作者：
Zhang, H.
影响因子：
3.4
作者：
CRAWFORD, DL;CRAWFORD, RL
通讯作者：
CRAWFORD, RL
影响因子：
3.4
作者：
Hofrichter, M
通讯作者：
Hofrichter, M