INDUCTION OF CELLULAR PROCESSES CONTAINING COLLAGENASE AND RETINOID BY INTEGRIN‐BINDING TO INTERSTITIAL COLLAGEN IN HEPATIC STELLATE CELL CULTURE
INDUCTION OF CELLULAR PROCESSES CONTAINING COLLAGENASE AND RETINOID BY INTEGRIN‐BINDING TO INTERSTITIAL COLLAGEN IN HEPATIC STELLATE CELL CULTURE
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通过整合素结合肝星状细胞培养物中的间质胶原来诱导含有胶原酶和类维生素A的细胞过程
DOI：
10.1006/cbir.1998.0234
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发表时间：
1998
影响因子：
3.9
通讯作者：
H. Senoo
中科院分区：
文献类型：
--
作者：
Mitsuru Sato;N. Kojima;M. Miura;K. Imai;H. Senoo
培养的肝星状细胞诱导伸长长，多极的细胞过程中使用的间质胶原凝胶作为基质，相比，扁平或圆形的细胞形状的聚苯乙烯表面或基质胶含有基底膜成分，分别。该过程的诱导被以下几种试剂抑制：（1）抗整联蛋白α2抗体;（2）寡肽DGEA，I型胶原分子中的整联蛋白结合序列;（3）渥曼青霉素，磷脂酰肌醇3激酶抑制剂。通过在I型胶原凝胶上培养，蛋白酪氨酸磷酸化在整个细胞包括细胞过程中增强。双重荧光染色显示，核心的过程中含有微管，而周边的过程包括纤维状肌动蛋白。因此，发现过程延伸依赖于整合素与I型胶原纤维的结合，随后是信号转导和细胞骨架组装。细胞过程包括间质胶原酶和含维生素A的脂滴。通过向培养基中添加视黄酯，脂滴和维生素A-自体荧光增加，表明肝星状细胞功能过程的重要作用。
Cultered hepatic stellate cells were induced to elongate long, multipolar cellular processes by interstitial collagen gel used as a substratum, as compared to flattened or round cell shapes on polystyrene surface or on Matrigel containing the basement membrane components, respectively. The process induction was inhibited by several reagents as follows: (1) anti‐integrin α2 antibody; (2) an oligopeptide, DGEA, an integrin‐binding sequence in type I collagen molecule; (3) wortmannin, a phosphatidylinositol 3‐kinase inhibitor. Protein tyrosine phosphorylation was enhanced throughout cells including cellular processes by culturing on type I collagen gel. Dual fluorescence staining showed that the core of the processes contained microtubules, whereas the periphery of the processes comprised fibrillar actin. Thus, the process extension was found to depend on integrin‐binding to type I collagen fibres, followed by signal transduction and cytoskeleton assembly. The cellular processes included interstitial collagenase and vitamin A‐containing lipid droplets. The lipid droplets and vitamin A‐autofluorescence were increased by retinyl acetate addition to the culture medium, suggesting an important role of processes in hepatic stellate cell function.
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DOI：
10.1073/pnas.82.24.8681
发表时间：
1985-12-01
影响因子：
11.1
作者：
FRIEDMAN, SL;ROLL, FJ;BISSELL, DM
通讯作者：
BISSELL, DM
通过在乳腺细胞中表达反义 α2 整合素 mRNA 来改变胶原依赖性粘附、运动和形态发生。
影响因子：
4
作者：
Keely,PJ;Fong,AM;Zutter,MM;Santoro,SA
通讯作者：
Santoro,SA
DOI：
10.1073/pnas.88.19.8392
发表时间：
1991-10-01
影响因子：
11.1
作者：
KORNBERG, LJ;EARP, HS;JULIANO, RL
通讯作者：
JULIANO, RL
胶原蛋白中α2β1整合素四肽识别序列的鉴定。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Staatz,WD;Fok,KF;Zutter,MM;Adams,SP;Rodriguez,BA;Santoro,SA
通讯作者：
Santoro,SA
细胞外基质调节细胞功能。
DOI：
10.1007/bf00861587
发表时间：
1993
期刊：
Pediatric nephrology (Berlin, Germany)
影响因子：
--
作者：
Schnaper,HW;Kleinman,HK
通讯作者：
Kleinman,HK