The Effects of Different Fungi on the IL-1β Expression in Mouse Dendritic Cells
The Effects of Different Fungi on the IL-1β Expression in Mouse Dendritic Cells
复制标题
不同真菌对小鼠树突状细胞 IL-1β 表达的影响
DOI：
10.5812/jjm.104089
复制
发表时间：
2020-09
期刊：
影响因子：
--
通讯作者：
帕丽达·阿布利孜
中科院分区：
文献类型：
--
作者：
张丽娟;王晓东;万学峰;哈地丽亚;帕丽达·阿布利孜
背景资料：侵袭性真菌感染因其病死率高而受到越来越多的关注，其中白色念珠菌和烟曲霉是系统性真菌感染最常见的病原菌，A. lentulus是最近分离鉴定的一种植物，被命名为A.烟熏..目的：本研究旨在探讨真菌毒力的浓度和时间依赖性关系以及白细胞介素-1 β（IL-1 β）水平的变化。方法：白色念珠菌、A.烟曲霉A.使用感染复数= 0、1、5、10和20单位的晶状体悬浮液处理小鼠树突细胞。在给药后2、6、12、24和48小时通过酶联免疫吸附测定（ELISA）测量IL-β水平。结果如下：感染复数、干预时间和IL-1 β因变量之间的主效应和交互作用显著。此外，有统计学意义的差异。只有C.白色念珠菌和A.给药后48h，白细胞介素1 β（IL-1 β）的表达明显增加。此外，A.烟曲霉诱导的细胞毒活性高于慢曲霉和C.白色念珠菌结论：本研究证明了三种真菌诱导的树突状细胞产生IL-1 β的浓度和时间依赖性关系。白色念珠菌和A.晶状体显示出缓慢的体外炎症诱导阶段。A.具有作用时间短、毒性强的特点。最后，A. lentulus比A.烟曲霉，其致炎时间相对较长。
Background: Invasive fungal infection received more and more attention because of its high mortality, Candida albicans and Aspergillus fumigatus is the most common pathogenic fungus for systematic fungal infection, A. lentulus was isolated and identified recently and named as a sister of A. fumigatus..Objectives: The current study aimed to explore the concentration and time-dependent relationships of the virulence of fungi due.to the change in the interleukin-1 beta (IL-1β) level..Methods: Candida albicans, A. fumigatus, and A. lentulus suspensions with a multiplicity of infection = 0, 1, 5, 10, and 20 units were used to treat mouse dendritic cells. The IL-β level was measured by enzyme-linked immunosorbent assay (ELISA) at 2, 6, 12, 24, and 48 hours after the treatment was administered..Results: The main effects and interactions between the multiplicity of infection, intervention duration, and the dependent variable of IL-1β were significant. Besides, there were statistically significant differences. Only C. albicans and A. lentulus could induce IL-1β 48 hours after administration. Furthermore, the production of IL-1β induced by A. fumigatus was higher than that induced by A.lentulus and C. albicans..Conclusions: This study demonstrated concentration- and time-dependent relationships in IL-1β production by dendritic cells induced by three types of fungi. Candida albicans and A. lentulus exhibited a slow phase-in in vitro inflammation induction. The inflammatory response induced by A. fumigatus in vitro has the characteristics of a short action time and a strong toxic effect. Finally, A.lentulus is less virulent than A. fumigatus, and its inflammation-inducing time is relatively longer.
登录
查看更多内容
影响因子：
3.7
作者：
Saïd-Sadier N;Padilla E;Langsley G;Ojcius DM
通讯作者：
Ojcius DM
DOI：
10.1016/j.bjm.2015.02.001
发表时间：
2016-01
期刊：
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
影响因子：
--
作者：
Zarrinfar H;Kaboli S;Dolatabadi S;Mohammadi R
通讯作者：
Mohammadi R
影响因子：
4.4
作者：
Kankkunen, Paivi;Teirila, Laura;Matikainen, Sampsa
通讯作者：
Matikainen, Sampsa
影响因子：
4.4
作者：
Kumar, Himanshu;Kumagai, Yutaro;Kawai, Taro
通讯作者：
Kawai, Taro
影响因子：
3
作者：
Montenegro, Graciela;Puch, Silvia Sanchez;Mujica, Maria T.
通讯作者：
Mujica, Maria T.