Rapid and Sensitive Determination of Timosaponin AIII in Rat Plasma by LC-MS/MS and Its Pharmacokinetic Application.
Rapid and Sensitive Determination of Timosaponin AIII in Rat Plasma by LC-MS/MS and Its Pharmacokinetic Application.
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LC-MS/MS 快速灵敏测定大鼠血浆中知母皂苷 AIII 及其药代动力学应用
DOI：
10.3390/ijms14023656
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发表时间：
2013-02-07
影响因子：
5.6
通讯作者：
Cai Z
中科院分区：
文献类型：
--
作者：
Liu Y;Pu Y;Zhang T;Ding Y;Wang B;Cai Z
建立了一种快速、灵敏、高选择性的液相色谱-串联质谱法（LC-MS/MS）测定大鼠血浆中知母皂苷A Ⅲ（TA Ⅲ）浓度的方法。TA-III和IS采用负离子电喷雾质谱仪在MRM模式下检测。校准曲线在11.14 - 1114 ng/mL浓度范围内呈线性，定量下限（LLOQ）为11.14 ng/mL。日内和日间精密度（RSD）均在10%以内，准确度范围为6.4%至9.1%。3种浓度下的提取回收率为92.3%~ 95.5%。经验证的方法成功地应用于监测TA-III在大鼠灌胃给药后的血浆中的浓度。用于估计TA-III大鼠经口给药组药代动力学参数的最佳拟合药代动力学模型为单室模型，重量为1/x2。
A rapid sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for determination of timosaponin AIII (TA-III) in rat plasma, using ginsenoside Re as an internal standard (IS). TA-III and the IS were detected in MRM mode with a negative ionization electrospray mass spectrometer. The calibration curves were linear over the concentration ranges from 11.14 to 1114 ng/mL and the lower limit of quantification (LLOQ) was 11.14 ng/mL. Intra-day and inter-day precisions (RSD) were within 10%, and accuracy ranged from 6.4% to 9.1%. The extraction recovery at three concentrations ranged from 92.3% to 95.5%. The validated method was successfully applied to monitor the concentrations of TA-III in rat plasma after intragastric administration. The best fit pharmacokinetic model to estimate the pharmacokinetic parameters was a single compartment model with weight of 1/x2 for oral administration groups of rats for TA-III.
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影响因子：
4.8
作者：
Lok, Chun-Nam;Sy, Lai-King;Che, Chi-Ming
通讯作者：
Che, Chi-Ming
影响因子：
3.7
作者：
King FW;Fong S;Griffin C;Shoemaker M;Staub R;Zhang YL;Cohen I;Shtivelman E
通讯作者：
Shtivelman E
影响因子：
11.2
作者：
Sy, Lai-King;Yan, Siu-Cheong;Che, Chi-Ming
通讯作者：
Che, Chi-Ming
DOI：
10.1016/j.jpba.2008.09.032
发表时间：
2008-12-15
影响因子：
3.4
作者：
Cai, Fei;Sun, Lianna;Chen, Wansheng
通讯作者：
Chen, Wansheng
影响因子：
3.6
作者：
Lee, Bomi;Jung, Kangsik;Kim, Dong-Hyun
通讯作者：
Kim, Dong-Hyun