Regulation of cell attachment and cell number by fibronectin and laminin
Regulation of cell attachment and cell number by fibronectin and laminin
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纤连蛋白和层粘连蛋白对细胞附着和细胞数量的调节
DOI：
10.1002/jcp.1041270318
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发表时间：
1986
影响因子：
5.6
通讯作者：
H. Kleinman
中科院分区：
文献类型：
--
作者：
V. Terranova;M. Aumailley;Leslie H. Sultan;G. Martin;H. Kleinman
我们研究了层粘连蛋白和纤维连接蛋白对一株小鼠上皮细胞和一株成人成纤维细胞在IV型胶原上附着和生长的影响。层粘连蛋白刺激表皮细胞的附着，纤维连接蛋白刺激成纤维细胞的附着。在高浓度(100μg/ml)时，附着蛋白改变了培养细胞的生长。表皮细胞在含层粘连蛋白的无纤维连接蛋白血清的培养液中生长较好。另一方面，成纤维细胞在含有血清和纤维连接蛋白的培养液中生长最好。这些数据表明，层粘连蛋白促进上皮细胞生长，而纤维连接蛋白促进成纤维细胞生长。当这些细胞在存在附着蛋白或其各自抗体的情况下共培养时，这一观察结果得到了证实。在成纤维细胞和上皮细胞共培养中加入层粘连蛋白，小鼠表皮细胞生长最好。成纤维细胞在抗层粘连蛋白抗体的存在下生长最好，在纤维连接蛋白抗体的存在下生长较差。因此，纤维连接蛋白和层粘连蛋白可能参与体内细胞群体的调节，并可能参与上皮-间充质的相互作用。
We have examined the effect of laminin and fibronectin on the attachment and growth on type IV collagen of a line of mouse epithelial cells and a strain of adult human fibroblasts. Laminin stimulated attachment of the epidermal cells and fibronectin stimulated fibroblast attachment. At high concentrations (100 μg/ml), the attachment proteins altered the growth of cells in culture. The epidermal cells grew better in media containing fibronectin‐free serum supplemented with laminin. Fibroblasts, on the other hand, grew best in media containing serum supplemented with fibronectin. These data suggest that laminin promotes epithelial cell growth whereas fibronectin promotes fibroblast growth. This observation was confirmed when these cells were cocultured in the presence of the attachment proteins or of their respective antibodies. The mouse epidermal cells grew best when laminin was added to cocultures of fibroblasts and epithelial cells. Fibroblasts grew best in the presence of antibody to laminin and poorly in the presence of antibody to fibronectin. Thus, fibronectin and laminin may participate in the regulation of cell populations in vivo and may be involved in epithelial‐mesenchymal interactions.
影响因子：
2.7
作者：
AKERS, RM;MOSHER, DF;LILIEN, JE
通讯作者：
LILIEN, JE
胚胎角膜上皮与外源胶原、层粘连蛋白和纤连蛋白的相互作用：内源蛋白质合成的作用。
影响因子：
2.7
作者：
Sugrue,SP;Hay,ED
通讯作者：
Hay,ED