Maternal aging affects oocyte resilience to carbonyl cyanide-m-chlorophenylhydrazone -induced mitochondrial dysfunction in cows.
Maternal aging affects oocyte resilience to carbonyl cyanide-m-chlorophenylhydrazone -induced mitochondrial dysfunction in cows.
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DOI：
10.1371/journal.pone.0188099
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Iwata H
中科院分区：
文献类型：
--
作者：
Kansaku K;Takeo S;Itami N;Kin A;Shirasuna K;Kuwayama T;Iwata H
线粒体质量控制对于维持细胞和卵母细胞的活力是重要的。此外，衰老影响许多细胞类型中的线粒体质量。在本研究中，我们研究了衰老如何影响卵母细胞线粒体的生物合成和退化，诱导线粒体功能障碍。从青年（21 ~ 45月龄）和老年（≥120月龄）奶牛的卵巢中收获卵丘卵母细胞复合体，用10 μM羰基氰化物-间氯苯腙（CCCP）或溶剂对照处理2小时，然后对卵丘卵母细胞复合体进行体外受精和培养。CCCP处理降低ATP含量和增加活性氧（ROS）水平在年轻和老年奶牛的卵母细胞。当CCCP处理的卵丘卵母细胞复合体随后培养19小时和/或进行受精时，在来自老年奶牛的卵母细胞中观察到卵母细胞中的高ROS水平和低囊胚发育率。此外，我们观察到年轻和老年奶牛之间的线粒体生物合成CCCP治疗的差异反应。CCCP处理增强线粒体生物合成伴随着SIRT 1表达的上调，在年轻的卵母细胞，但不是老年，奶牛。总之，衰老影响线粒体质量控制和CCCP诱导的线粒体功能障碍后卵母细胞的恢复。
Mitochondrial quality control is important for maintaining cellular and oocyte viability. In addition, aging affects mitochondrial quality in many cell types. In the present study, we examined how aging affects oocyte mitochondrial biogenesis and degeneration in response to induced mitochondrial dysfunction. Cumulus oocyte complexes were harvested from the ovaries of young (21‒45 months) and aged (≥120 months) cows and treated for 2 hours with 10 μM carbonyl cyanide-m- chlorophenylhydrazone (CCCP), or a vehicle control, after which cumulus oocyte complexes were subjected to in vitro fertilization and culture. CCCP treatment reduced ATP content and increased reactive oxygen species (ROS) levels in the oocytes of both young and aged cows. When CCCP-treated cumulus oocyte complexes were subsequently cultured for 19 hours and/or subjected to fertilization, high ROS levels in oocytes and a low rate of blastocyst development was observed in oocytes derived from aged cows. In addition, we observed differential responses in mitochondrial biogenesis to CCCP treatment between young and aged cows. CCCP treatment enhanced mitochondrial biogenesis concomitant with upregulation of SIRT1 expression in oocytes of young, but not aged, cows. In conclusion, aging affects mitochondrial quality control and recuperation of oocytes following CCCP-induced mitochondrial dysfunction.
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影响因子：
29
作者：
Tilly JL;Sinclair DA
通讯作者：
Sinclair DA
影响因子：
7.8
作者：
Sosulski ML;Gongora R;Danchuk S;Dong C;Luo F;Sanchez CG
通讯作者：
Sanchez CG
Parkin对泛素 - 蛋白酶体系统的广泛激活对于线粒体至关重要。
影响因子：
3.5
作者：
Chan NC;Salazar AM;Pham AH;Sweredoski MJ;Kolawa NJ;Graham RL;Hess S;Chan DC
通讯作者：
Chan DC
影响因子：
3.7
作者：
Simsek-Duran F;Li F;Ford W;Swanson RJ;Jones HW Jr;Castora FJ
通讯作者：
Castora FJ
DOI：
10.1083/jcb.200809125
发表时间：
2008-12-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Narendra D;Tanaka A;Suen DF;Youle RJ
通讯作者：
Youle RJ