Age-associated metabolic and morphologic changes in mitochondria of individual mouse and hamster oocytes.
Age-associated metabolic and morphologic changes in mitochondria of individual mouse and hamster oocytes.
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DOI：
10.1371/journal.pone.0064955
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Castora FJ
中科院分区：
文献类型：
--
作者：
Simsek-Duran F;Li F;Ford W;Swanson RJ;Jones HW Jr;Castora FJ
在人类卵母细胞中，与其他哺乳动物卵子一样，妊娠潜力存在显著差异，约20%的卵母细胞-精子相遇导致妊娠。这种成功受精的频率随着卵母细胞年龄的增长而降低。这种低比例的富有成效的耦合似乎是线粒体结构和功能的变化的影响。在这项研究中，我们研究了仓鼠（Mesocricetus auratus）和小鼠（Mus musculus）的卵子线粒体生物合成模型，了解衰老对哺乳动物卵母细胞内线粒体结构和能量产生的影响。在激素刺激后，从卵泡中收集来自总共25只年轻和年老小鼠和仓鼠的单个中期II卵母细胞，并制备用于生化或结构分析。在年轻和年老动物的单个卵母细胞内测定三磷酸腺苷水平和线粒体DNA数量。在老年仓鼠，卵母细胞腺苷三磷酸水平和线粒体DNA分子分别减少35.4%和51.8%。三磷酸腺苷和线粒体基因组分别减少了38.4%和44%，在老年小鼠卵母细胞中也观察到。透射电镜（TEM）分析表明，老龄鼠卵母细胞的线粒体和胞质层状结构发生了明显的变化。在小鼠和仓鼠中，老化卵母细胞中三磷酸腺苷的减少与线粒体DNA分子和线粒体数量的类似减少相关。随着年龄的增长，小鼠和仓鼠的线粒体发生了明显的形态学变化，包括线粒体空泡化、嵴改变和细胞质层的变化。
In human oocytes, as in other mammalian ova, there is a significant variation in the pregnancy potential, with approximately 20% of oocyte-sperm meetings resulting in pregnancies. This frequency of successful fertilization decreases as the oocytes age. This low proportion of fruitful couplings appears to be influenced by changes in mitochondrial structure and function. In this study, we have examined mitochondrial biogenesis in both hamster (Mesocricetus auratus ) and mouse (Mus musculus) ova as models for understanding the effects of aging on mitochondrial structure and energy production within the mammalian oocyte. Individual metaphase II oocytes from a total of 25 young and old mice and hamsters were collected from ovarian follicles after hormone stimulation and prepared for biochemical or structural analysis. Adenosine triphosphate levels and mitochondrial DNA number were determined within individual oocytes from young and old animals. In aged hamsters, oocyte adenosine triphosphate levels and mitochondrial DNA molecules were reduced 35.4% and 51.8%, respectively. Reductions of 38.4% and 44% in adenosine triphosphate and mitochondrial genomes, respectively, were also seen in aged mouse oocytes. Transmission electron microscopic (TEM) analysis showed that aged rodent oocytes had significant alterations in mitochondrial and cytoplasmic lamellae structure. In both mice and hamsters, decreased adenosine triphosphate in aged oocytes is correlated with a similar decrease in mtDNA molecules and number of mitochondria. Mitochondria in mice and hamsters undergo significant morphological change with aging including mitochondrial vacuolization, cristae alterations, and changes in cytoplasmic lamellae.
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影响因子：
3.8
作者：
El Shourbagy, SH;Spikings, EC;St John, JC
通讯作者：
St John, JC
小鼠卵母细胞线粒体功能受损与受控卵巢过度刺激和体外成熟有关
影响因子：
1.9
作者：
Ge, Hongshan;Tollner, Theodore L.;Dong, Qiaoxiang
通讯作者：
Dong, Qiaoxiang
影响因子：
3.6
作者：
Stojkovic, M;Machado, SA;Wolf, E
通讯作者：
Wolf, E
影响因子：
3.1
作者：
Kushnir, Vitaly A.;Ludaway, Tomika;Lewis, William
通讯作者：
Lewis, William
DOI：
10.1530/jrf.0.0350301
发表时间：
1973-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
QUINN, P;WALES, RG
通讯作者：
WALES, RG