Enhancement of adenovirus delivery after ultrasound-stimulated therapy in a cancer model.
Enhancement of adenovirus delivery after ultrasound-stimulated therapy in a cancer model.
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DOI：
10.1016/j.ultrasmedbio.2013.07.017
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发表时间：
2013-12
影响因子：
2.9
通讯作者：
Hoyt, Kenneth
中科院分区：
文献类型：
--
作者：
Sorace, Anna G.;Warram, Jason M.;Mahoney, Marshall;Zinn, Kurt R.;Hoyt, Kenneth
Improving the efficiency of adenovirus (Ad) delivery to target tissues has the potential to advance the translation of cancer gene therapy. Ultrasound (US)-stimulated therapy uses microbubbles (MBs) exposed to low-intensity US energy to improve localized delivery. We hypothesize that US-stimulated gene therapy can improve Ad infection in a primary prostate tumor through enhanced tumor uptake and retention of the Ad vector. In vitro studies were performed to analyze the degree of Ad infectivity after application of US-stimulated gene therapy. A luciferase-based Ad on a ubiquitous cytomegalovirus (CMV) promoter (Ad5/3-CMV-Luc) was used in an animal model of prostate cancer (bilateral tumor growth) to evaluate Ad transduction efficiency after US-stimulated therapy. Bioluminescence imaging was employed for In vivo analysis to quantify Ad infection within the tumor. In vitro studies revealed no difference in Ad transduction between groups receiving US-stimulated therapy using high, low or sham US intensity exposures at various multiplicities of infection (MOIs) (p = 0.80). In vivo results indicated that tumors receiving US-stimulated therapy after intra-tumoral injection of Ad5/3-CMV-Luc (1 × 106 plaque-forming units) exhibited a 95.1% enhancement in tumor delivery compared with control tumors receiving sham US (p = 0.03). US-stimulated therapy has significant potential to immediately affect Ad-based cancer gene therapy by improving virus bioavailability in target tissues.
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影响因子：
11.2
作者：
McDannold N;Arvanitis CD;Vykhodtseva N;Livingstone MS
通讯作者：
Livingstone MS
DOI：
10.1016/j.euje.2004.02.001
发表时间：
2004-08-01
期刊：
European journal of echocardiography : the journal of the Working Group on Echocardiography of the European Society of Cardiology
影响因子：
--
作者：
Dijkmans, P A;Juffermans, L J M;Kamp, O
通讯作者：
Kamp, O
DOI：
10.1016/j.jconrel.2010.06.012
发表时间：
2010-11-20
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Böhmer MR;Chlon CHT;Raju BI;Chin CT;Shevchenko T;Klibanov AL
通讯作者：
Klibanov AL
影响因子：
2.9
作者：
Casey, Garrett;Cashman, James P.;O'Sullivan, Gerald C.
通讯作者：
O'Sullivan, Gerald C.
影响因子：
6.4
作者：
Borovjagin, AV;Krendelchtchikov, A;Curiel, DT
通讯作者：
Curiel, DT