Antigen 84, an Effector of Pleiomorphism in Mycobacterium smegmatis
Antigen 84, an Effector of Pleiomorphism in Mycobacterium smegmatis
复制标题
抗原 84，耻垢分枝杆菌多形性效应子
作者：
Liem D. Nguyen;Nicole Scherr;J. Gatfield;A. Walburger;J. Pieters;C. Thompson
摘要虽然在大多数杆状细菌中，形态学是基于MreB样蛋白，其形成用于细胞壁生物合成的肌动蛋白样细胞骨架支架，但在放线菌例如分枝杆菌物种中决定更灵活的杆状形状的因素是未知的。在这里，我们表明，耻垢分枝杆菌蛋白同源真细菌DivIVA样蛋白，包括M。结核抗原84（Ag 84），对称地定位于两极和隔膜处的肽聚糖生物合成中心。受控基因破坏实验表明，编码Ag84的基因，wag31，是必不可少的，当过表达，细胞变得更长，更宽，与Ag84不对称地分布在一个极点。许多细胞变得非常大，保龄球针形细胞的体积增加了80倍。在这些细胞中，Ag84主要积累在球根极，这显然是由不受控制的细胞壁扩张产生的。在一些细胞中，Ag84与细胞壁扩展（芽）的特殊位点相关，这些位点进化成分支。M. Bovis BCG Ag 84能够在体外形成寡聚体，这可能反映了其在体内的超结构。这些数据表明，Ag84在多形放线菌的细胞分裂和调节细胞形状的作用。
ABSTRACT While in most rod-shaped bacteria, morphology is based on MreB-like proteins that form an actin-like cytoskeletal scaffold for cell wall biosynthesis, the factors that determine the more flexible rod-like shape in actinobacteria such as Mycobacterium species are unknown. Here we show that a Mycobacterium smegmatis protein homologous to eubacterial DivIVA-like proteins, including M. tuberculosis antigen 84 (Ag84), localized symmetrically to centers of peptidoglycan biosynthesis at the poles and septa. Controlled gene disruption experiments indicated that the gene encoding Ag84, wag31, was essential; when overexpressed, cells became longer and wider, with Ag84 asymmetrically distributed at one pole. Many became grossly enlarged, bowling-pin-shaped cells having up to 80-fold-increased volume. In these cells, Ag84 accumulated predominantly at a bulbous pole that was apparently generated by uncontrolled cell wall expansion. In some cells, Ag84 was associated with exceptional sites of cell wall expansion (buds) that evolved into branches. M. bovis BCG Ag84 was able to form oligomers in vitro, perhaps reflecting its superstructure in vivo. These data suggested a role for Ag84 in cell division and modulating cell shape in pleiomorphic actinobacteria.
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影响因子：
10.5
作者：
Kang, CM;Abbott, DW;Husson, RN
通讯作者：
Husson, RN
DOI：
10.1073/pnas.96.9.4971
发表时间：
1999-04-27
影响因子：
11.1
作者：
Raskin, DM;de Boer, PAJ
通讯作者：
de Boer, PAJ
影响因子：
2.8
作者：
Bardarov, S;Bardarov, S;Jacobs, WR
通讯作者：
Jacobs, WR
影响因子：
16
作者：
Bernhardt, TG;de Boer, PAJ
通讯作者：
de Boer, PAJ