Monitoring the Process of Endostar-Induced Tumor Vascular Normalization by Non-contrast Intravoxel Incoherent Motion Diffusion-Weighted MRI.
Monitoring the Process of Endostar-Induced Tumor Vascular Normalization by Non-contrast Intravoxel Incoherent Motion Diffusion-Weighted MRI.
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通过非对比体素内不相干运动扩散加权 MRI 监测恩度诱导的肿瘤血管正常化过程
DOI：
10.3389/fonc.2018.00524
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发表时间：
2018
影响因子：
4.7
通讯作者：
Pan Y
中科院分区：
文献类型：
--
作者：
Pan JH;Zhu S;Huang J;Liang J;Zhang D;Zhao X;Ding H;Qin L;Shi C;Luo L;Pan Y
肿瘤血管归一化是抗肿瘤血管生成的一个新概念，归一化窗口被认为是提高放化疗效果的契机。然而，目前尚缺乏一种无创监测肿瘤血管正常化过程的方法。体素内非相干运动弥散加权磁共振成像(IVIM DW-MRI)是一种不需要外源性造影剂就能有效评价肿瘤微血流灌注的新方法。但其在肿瘤血管正常化监测中的作用仍需进一步研究。本研究通过Endostar治疗建立了CT26结肠癌荷瘤小鼠肿瘤血管正常化模型。治疗后不同时间分别采用IVIM、DW-MRI和免疫荧光技术检测肿瘤血管正常化过程。在治疗后第4、6、8、10天，Endostar组的D*值显著高于对照组，而在第6、8天，Endostar组的f值显著高于对照组。此外，通过对组织学参数的分析，我们证实Endostar组从治疗后第4天开始诱导CT26肿瘤血管正常化窗口，该窗口持续6天。此外，我们发现D*和f值与周细胞覆盖率(r分别为0.469和0.504；P<；0.001)和相对灌注量(r分别为0.424和0.457；P<；0.001)有很好的相关性。综上所述，我们的研究结果表明，IVIM DW-MRI有可能成为监测Endostar诱导的肿瘤血管正常化的一种无创方法。
Tumor vascular normalization has been proposed as a new concept in anti-tumor angiogenesis, and the normalization window is considered as an opportunity to increase the effect of chemoradiotherapy. However, there is still a lack of a non-invasive method for monitoring the process of tumor vascular normalization. Intravoxel incoherent motion diffusion-weighted magnetic resonance imaging (IVIM DW-MRI) is an emerging approach which can effectively assess microperfusion in tumors, without the need for exogenous contrast agents. However, its role in monitoring tumor vascular normalization still needs further study. In this study, we established a tumor vascular normalization model of CT26 colon-carcinoma-bearing mice by means of Endostar treatment. We then employed IVIM DW-MRI and immunofluorescence to detect the process of tumor vascular normalization at different times after treatment. We found that the D* values of the Endostar group were significantly higher than those of the control group on days 4, 6, 8, and 10 after treatment, and the f values of the Endostar group were significantly higher than those of the control group on days 6 and 8. Furthermore, we confirmed through analysis of histologic parameters that Endostar treatment induced the CT26 tumor vascular normalization window starting from day 4 after treatment, and this window lasted for 6 days. Moreover, we found that D* and f values were well correlated with pericyte coverage (r = 0.469 and 0.504, respectively; P < 0.001, both) and relative perfusion (r = 0.424 and 0.457, respectively; P < 0.001, both). Taken together, our findings suggest that IVIM DW-MRI has the potential to serve as a non-invasive approach for monitoring Endostar-induced tumor vascular normalization.
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