Alveolar and tissue phospholipids of rat lung.
Alveolar and tissue phospholipids of rat lung.
复制标题
大鼠肺的肺泡和组织磷脂。
DOI：
10.1620/tjem.108.253
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
T. Akino
中科院分区：
文献类型：
--
作者：
N. Toshima;T. Akino
使用硅酸和 DEAE-纤维素柱色谱法以及薄层色谱法和气相色谱法研究大鼠肺组织和肺泡洗液中磷脂的化学性质，以确定主要磷脂组分的脂肪酸组成。研究发现，通过肺灌洗获得的肺泡磷脂在几个方面与肺组织中的不同。两种来源的主要磷脂是卵磷脂，其占肺泡磷脂的 68% 和肺组织磷脂的 52%。肺泡卵磷脂含有比肺组织高得多的不饱和物质。肺泡洗液中脂质 X1、X2 和 X3 的比例较高。从完整脂质以及脱酰化产物的Rf值数据以及DEAE-纤维素的洗脱模式来看，X3似乎与心磷脂相同。酸性脂质X2似乎可能是磷脂酰甘油。无法鉴定脂质 Xl。目前的结果表明，卵磷脂和三种酸性磷脂比其他脂质（如中性脂质、磷脂酰乙醇胺、磷脂酰丝氨酸、磷脂酰肌醇和鞘磷脂）更优先分泌到肺泡腔中。
The chemical nature of phospholipids in rat lung tissue and alveolar wash was studied using column chromatographies on silicic acid and DEAE-cellulose and thin-layer chromato-graphy followed by gas chromatography to determine the fatty acid composition of the major phospholipid fractions. It was found that alveolar phospholipids obtained by lung lavage differed from those in lung tissue in several aspects. Predominant phospholipid from both sources was lecithin which composed 68% of alveolar phospholipids and 52% of lung tissue phospholipids. Alveolar lecithin contained much higher disaturated species than lung tissue. Lipids X1, X2 and X3 were found in higher proportions in alveolar wash. The X3 seemed to be identical with cardiolipin from the data of Rf values of intact lipids as well as deacylated products, and elution pattern with DEAE-cellulose. The acidic lipid X2 seemed to be probably phosphatidylglycerol. Lipid Xl could not be identified. The present results suggested that lecithin and the three acidic phospholipids could be secreted more preferentially into alveolar cavity than other lipids, such as neutral lipids, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol and sphingomyelin.
影响因子：
6.5
作者：
W. R. Morrison;L. Smith
通讯作者：
W. R. Morrison;L. Smith