Tangle analysis of Xer recombination reveals only three solutions, all consistent with a single three-dimensional topological pathway.
Tangle analysis of Xer recombination reveals only three solutions, all consistent with a single three-dimensional topological pathway.
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Xer 重组的缠结分析仅揭示了三种解决方案，均与单个三维拓扑路径一致。
DOI：
10.1016/j.jmb.2004.11.055
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发表时间：
2005
影响因子：
5.6
通讯作者：
D. Sumners
中科院分区：
文献类型：
--
作者：
M. Vázquez;S. Colloms;D. Sumners
在环状未打结DNA分子上直接重复psi位点的Xer重组产物是右手四节索烃。在这里，我们使用缠结方程来分析与Xer重组在psi的拓扑变化。这种数学方法允许计算与实验数据一致的所有可能的拓扑路径。我们给出了一个严格的数学证明，在合理的生物学假设下，缠结方程只有三个解。其中一个解决方案对应于一个突触复合体与重组核心网站的反平行对齐，其他两个对应于平行对齐的核心。我们表明，所有三个解决方案可以统一到一个单一的三维模型Xer重组。因此，这三个不同的数学解不一定代表不同的三维路径，在这种情况下，这三个不同的缠结解是相同三维构型的不同平面投影。
The product of Xer recombination at directly repeated psi sites on a circular unknotted DNA molecule is a right-hand four-noded catenane. Here, we use tangle equations to analyze the topological changes associated with Xer recombination at psi. This mathematical method allows computation of all possible topological pathways consistent with the experimental data. We give a rigorous mathematical proof that, under reasonable biological assumptions, there are only three solutions to the tangle equations. One of the solutions corresponds to a synaptic complex with antiparallel alignment of recombination core sites, the other two correspond to parallel alignment of cores. We show that all three solutions can be unified into a single three-dimensional model for Xer recombination. Thus the three distinct mathematical solutions do not necessarily represent distinct three-dimensional pathways, and in this case the three distinct tangle solutions are different planar projections of the same three-dimensional configuration.
DOI：
10.1101/sqb.1984.049.01.045
发表时间：
1984-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
COZZARELLI, NR;KRASNOW, MA;WHITE, JH
通讯作者：
WHITE, JH
影响因子：
56.9
作者：
WASSERMAN, SA;DUNGAN, JM;COZZARELLI, NR
通讯作者：
COZZARELLI, NR