SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse.
SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse.
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DOI：
10.1038/s41467-022-35465-1
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发表时间：
2022-12-21
影响因子：
16.6
通讯作者：
Kanai, Yoshiakira
中科院分区：
文献类型：
--
作者：
Uchida, Aya;Imaimatsu, Kenya;Suzuki, Honoka;Han, Xiao;Ushioda, Hiroki;Uemura, Mami;Imura-Kishi, Kasane;Hiramatsu, Ryuji;Takase, Hinako M.;Hirate, Yoshikazu;Ogura, Atsuo;Kanai-Azuma, Masami;Kudo, Akihiko;Kanai, Yoshiakira
哺乳动物睾丸中的精小管（STs）通过支持阀（SV）连接到睾丸网（RT）。在STs中产生的精子被释放到管状管腔液中，并被动地通过SV进入RT。然而，RT和SV的生理功能尚不清楚。在这里，我们鉴定了Sox17在RT上皮中的表达。在rt特异性Sox17条件敲除（Sox17- cko）的雄性小鼠中，SV阀在青春期前被破坏。这导致RT液回流到STs中，导致未成熟精子异常脱离。Sox17-cKO小鼠的RT降低了各种生长因子基因的表达水平，可能支持SV的形成。当移植到Sox17+ RT旁时，Sox17- cko小鼠的支持细胞重建了SV，并支持了STs中正常的精子发生。这项研究强调了RT作为Sox17的下游作用在小鼠睾丸SV瓣膜形成和精子发生中的新颖和意想不到的调节作用。一个被称为Sertoli瓣膜（SV）的瓣膜状结构通过调节小鼠睾丸中的定向流体流动来支持精子发生。SV的形成是由邻近的SOX17 +睾丸网（RT）支持的。本研究强调了RT和SV在精子发生中的重要作用。
Seminiferous tubules (STs) in the mammalian testes are connected to the rete testis (RT) via a Sertoli valve (SV). Spermatozoa produced in the STs are released into the tubular luminal fluid and passively transported through the SV into the RT. However, the physiological functions of the RT and SV remain unclear. Here, we identified the expression of Sox17 in RT epithelia. The SV valve was disrupted before puberty in RT-specific Sox17 conditional knockout (Sox17-cKO) male mice. This induced a backflow of RT fluid into the STs, which caused aberrant detachment of immature spermatids. RT of Sox17-cKO mice had reduced expression levels of various growth factor genes, which presumably support SV formation. When transplanted next to the Sox17+ RT, Sertoli cells of Sox17-cKO mice reconstructed the SV and supported proper spermiogenesis in the STs. This study highlights the novel and unexpected modulatory roles of the RT in SV valve formation and spermatogenesis in mouse testes, as a downstream action of Sox17. A valve-like structure called this Sertoli valve (SV) supports spermatogenesis by modulating the directional fluid flow in mouse testis. The SV formation is supported by its neighboring SOX17 + rete testis (RT). This study highlights the essential roles of RT and SV in spermatogenesis.
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影响因子：
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作者：
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通讯作者：
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作者：
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