Redox changes in cat brain cytochrome-c oxidase after blood-fluorocarbon exchange.
Redox changes in cat brain cytochrome-c oxidase after blood-fluorocarbon exchange.
复制标题
血液-氟碳交换后猫脑细胞色素 C 氧化酶的氧化还原变化。
DOI：
10.1152/ajpheart.1990.258.6.h1706
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Traystman,RJ
中科院分区：
文献类型：
--
作者：
Ferrari,M;Hanley,DF;Wilson,DA;Traystman,RJ
本文应用快速扫描近红外光谱法（730-960 nm），通过血液-全氟化学乳剂（Oxypherol）交换，测定猫脑细胞色素C氧化酶铜带。光谱进行前，期间和之后的交换输血的动物与保存的体感诱发电位和微球确定的脑血流量。剩余的血红蛋白（小于对照的4%）转化为在该光谱区域不吸收的碳氧血红蛋白。高碳酸血症状态（8%CO2 - 92%O2）和死后之间的差异光谱表明存在以820-845 nm为中心的宽吸收带，这可能归因于细胞色素c氧化酶的氧化低电位铜离子（CuA）。然而，我们无法通过向吸入气体混合物中添加CO2来进一步氧化该条带，但这种不一致性可能是由于该制剂中存在接近最大的脑血流量水平。CO2引起的细胞色素氧化通常归因于向组织输送的O2增加，继发于脑灌注增加。我们无法进一步增加脑血流量。相反，细胞色素带可以减少通过降低分数O2浓度和诱导循环停止。光谱数据支持的假设，这是可能的，以量化的细胞色素c氧化酶铜带在近红外光谱区。
Rapid scanning near-infrared spectroscopy (730-960 nm) was utilized to determine cat brain cytochrome-c oxidase copper band by blood-perfluorochemical emulsion (Oxypherol) exchange. Spectra were carried out before, during, and after the exchange transfusion on animals with preserved somatosensory-evoked potentials and microsphere-determined cerebral blood flow. Remaining hemoglobin (less than 4% of control) was converted to carboxyhemoglobin that does not absorb in this spectral region. Difference spectra, between an hypercapnic status (8% CO2-92% O2) and postmortem, demonstrated the presence of a broad absorption band centered around 820-845 nm that could be attributed to the oxidized low potential copper ion (CuA) of cytochrome-c oxidase. However, we were unable to further oxidize this band by adding CO2 to the inspired gas mixture, but this inconsistency may be due to the near-maximal cerebral blood flow levels present in this preparation. Cytochrome oxidation by CO2 is normally attributed to increased O2 delivery to the tissue, secondary to an increased cerebral perfusion. We were unable to induce further increases in cerebral blood flow. In contrast, the cytochrome band could be reduced both by lowering fractional O2 concentration and by inducing circulatory arrest. The spectral data support the hypothesis that it is possible to quantify the cytochrome-c oxidase copper band in the near-infrared spectral region.
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作者：
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DOI：
10.21236/ada119814
发表时间：
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期刊：
The American journal of physiology
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作者：
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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