Zebrafish Ski7 tunes RNA levels during the oocyte-to-embryo transition.
Zebrafish Ski7 tunes RNA levels during the oocyte-to-embryo transition.
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斑马鱼Ski7在卵母细胞到胚胎的转变过程中调节RNA水平。
DOI：
10.1371/journal.pgen.1009390
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发表时间：
2021-03
期刊：
影响因子：
4.5
通讯作者：
Pauli A
中科院分区：
文献类型：
--
作者：
Cabrera-Quio LE;Schleiffer A;Mechtler K;Pauli A
在卵母细胞到胚胎的转变过程中，基因表达的转录后调控是至关重要的，这是一个以缺乏核转录为特征的高度动态的过程。因此，RNA含量的变化完全取决于RNA的降解。虽然已经确定了在胚胎发生过程中促进RNA衰退的几种机制，但还不清楚哪些机制有助于重塑母体转录组。在这里，我们重点研究了斑马鱼中的降解因子Ski7。纯合的ski7突变鱼有更高比例的劣质卵和无法超过单细胞阶段发育的卵。与Ski7参与重塑母体RNA内容的想法一致，转录组图谱在没有Ski7的情况下发现了数百个调控不当的mRNAs。此外，上调基因在野生型中的表达普遍较低，这表明Ski7保持着这一基因亚集的低转录水平。最后，GO浓缩和错误调节因子的蛋白质组学分析表明，Ski7参与了氧化还原过程的调节。Ski7突变胚胎对还原压力的抵抗力增强，在实验上证实了这一点。我们的结果首次揭示了脊椎动物Ski7在卵母细胞到胚胎的转变过程中作为转录后调节因子的生理作用。在早期胚胎发育期间，所有的细胞过程都由卵子中沉积的母体产物调节。其中一种产物是RNA，它是合成蛋白质的模板。随着胚胎在细胞数量和大小上的增长，需要去除RNA。虽然已经确定了许多调节胚胎中RNA降解的成分和途径，但完整的一套机制仍然不清楚。在这里，我们描述了超级杀手7(Ski7)蛋白的功能，已知该蛋白参与了酵母中RNA的降解。我们利用高通量技术，在Ski7存在和缺失的情况下，对斑马鱼从卵到胚胎过渡过程中RNA的状态进行了分析。斑马鱼中Ski7的缺失导致数百个转录本的错误调节，降低卵子质量，并导致雌性鱼类可能不育。总体而言，Ski7在脊椎动物中是一个以前未被认识的因子，在从卵到胚胎的过渡过程中对RNA降解的调节非常重要。
Post-transcriptional regulation of gene expression is crucial during the oocyte-to-embryo transition, a highly dynamic process characterized by the absence of nuclear transcription. Thus, changes to the RNA content are solely dependent on RNA degradation. Although several mechanisms that promote RNA decay during embryogenesis have been identified, it remains unclear which machineries contribute to remodeling the maternal transcriptome. Here, we focused on the degradation factor Ski7 in zebrafish. Homozygous ski7 mutant fish had higher proportions of both poor quality eggs and eggs that were unable to develop beyond the one-cell stage. Consistent with the idea that Ski7 participates in remodeling the maternal RNA content, transcriptome profiling identified hundreds of misregulated mRNAs in the absence of Ski7. Furthermore, upregulated genes were generally lowly expressed in wild type, suggesting that Ski7 maintains low transcript levels for this subset of genes. Finally, GO enrichment and proteomic analyses of misregulated factors implicated Ski7 in the regulation of redox processes. This was confirmed experimentally by an increased resistance of ski7 mutant embryos to reductive stress. Our results provide first insights into the physiological role of vertebrate Ski7 as a post-transcriptional regulator during the oocyte-to-embryo transition. During early embryo development, all cellular processes are regulated by maternal products deposited in the egg. One of such products is RNA, which serves as a template for the synthesis of proteins. As the embryo grows in the number of cells and size, RNAs need to be removed. Although many components and pathways that regulate RNA degradation in the embryo have been identified, the complete set of mechanisms are still unclear. Here, we characterize the function of the superkiller 7 (Ski7) protein, known to be involved in RNA degradation in yeast. We take advantage of high-throughput technologies to profile the status of RNAs during the transition from the egg to the embryo in zebrafish in the presence and abscense of Ski7. Absence of Ski7 in zebrafish leads to misregulation of hundreds of transcripts, decreases egg quality, and causes presumptive subfertility in female fish. Overall, Ski7 is a previously unrecognized factor in vertebrates that is important for the regulation of RNA degradation during the transition from the egg to the embryo.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
64.5
作者：
Halbach, Felix;Reichelt, Peter;Conti, Elena
通讯作者：
Conti, Elena
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
影响因子：
4.4
作者：
Dorfer, Viktoria;Pichler, Peter;Stranzl, Thomas;Stadlmann, Johannes;Taus, Thomas;Winkler, Stephan;Mechtler, Karl
通讯作者：
Mechtler, Karl
DOI：
10.1126/science.aat7113
发表时间：
2018-09-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Herberg S;Gert KR;Schleiffer A;Pauli A
通讯作者：
Pauli A