Engineering of the chloroaniline-catabolic plasmid pDCA-1 and its potential for genetic bioaugmentation
Engineering of the chloroaniline-catabolic plasmid pDCA-1 and its potential for genetic bioaugmentation
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氯苯胺分解代谢质粒 pDCA-1 的工程及其遗传生物增强的潜力
DOI：
10.1016/j.ibiod.2022.105435
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发表时间：
2022-08
影响因子：
4.8
通讯作者：
Kai Chen
中科院分区：
文献类型：
--
作者：
Zhuang Ke;Shen Wang;Weixian Dai;Weibin Jia;Yang Mu;Ji;ong Jiang;Kai Chen
质粒通过水平转移传播污染物分解代谢基因，并有助于宿主微生物群落的分解代谢潜力。最近，对结合的分解代谢质粒进行遗传生物增强被认为是一种有效的、新的污染部位长期生物修复的方法。在本研究中，我们从Achromobacter sp.中构建了IncP-1β-2亚群表达载体pDCA-1。ANB-1在遗传生物增强中的潜在应用。在广泛的宿主范围的pPDCA-1上发现了一个负责氯代苯胺脱氨制氯代邻苯二酚的dcaA1A2B簇和一个用于氯代邻苯二酚环裂解的1，2-双加氧酶基因(Ccdc)。在pDCA-1的辅助区插入一个酰胺酶基因(phh或tccA2)，大大扩展了它的分解底物谱，从氯苯胺(3-氯苯胺和3，4-二氯苯胺)到除草剂(利百隆、丙胺、丙草胺和敌草胺)、抑菌剂(三氯卡万)、植物调节剂(氟虫隆)和杀虫剂(敌百隆)。利用高通量细胞分选和基于16S rRNA基因的扩增片段测序的优势，我们描述了土壤细菌群落中工程质粒PDCA-1-GFP-PHH的细菌受体多样性，表明PDCA-1衍生物在自然环境中对各种革兰氏阴性甚至阳性菌株具有良好的接合转移能力。综上所述，PDCA-1工程载体在氯苯胺及其衍生物污染部位的遗传生物强化方面具有很大的潜力。鉴定了IncP-1β亚群中的一个新成员--PDCA-1。·PDCA-1编码含有dcaA1A2B和CCDC基因的氯苯胺分解代谢途径。·PHH/tccA2的引入极大地扩大了PDCA-1的分解底物范围。·PDCA-1具有广泛的宿主范围，包括革兰氏阴性和阳性菌株。·PDCA-1衍生物在遗传生物增强方面具有巨大的潜力。
Plasmids disseminate pollutant-catabolic genes through horizontal transfer and contribute to the catabolic potential of the host microbial community. Genetic bioaugmentation of conjugative catabolic plasmids has recently been considered an effective and novel approach for long-term bioremediation of contaminated sites. In this study, we engineered an IncP-1 β -2 subgroup plasmid pDCA-1 from Achromobacter sp. ANB-1 for its potential application in genetic bioaugmentation. A dcaA1A2B cluster responsible for the deamination of chloroaniline to chlorocatechol and a 1,2-dioxygenase gene ( ccdC ) for the ring cleavage of chlorocatechol were found to locate on the broad host range plasmid pDCA-1. Insertion of an amidase gene ( phh or tccA2 ) at the accessory region of plasmid pDCA-1 greatly expanded its catabolic substrate spectrum from chloroaniline (3-chloroaniline and 3,4-dichloroaniline) to herbicides (linuron, propanil, propham, and chlorpropham), bacteriostatic agent (triclocarban), plant regulator (forchlorfenuron), and insecticide (diflubenzuron). Taking advantages of high-throughput cell sorting and 16S rRNA gene-based amplicon sequencing, we depicted the diversity of bacterial recipients for the engineered plasmid pDCA-1- gfp-phh in a soil bacterial consortium, showing an excellent conjugative transfer capacity of the pDCA-1 derivative to various Gram negative and even positive strains in natural environment. All in all, the engineered plasmid pDCA-1 had a great potential in genetic bioaugmentation of the sites contaminated with chloroanilines and their derivatives. • pDCA-1, a new member among IncP-1 β subgroup plasmids, was characterized. • pDCA-1 encoded chloroaniline-catabolic pathway containning dcaA1A2B and ccdC genes. • Introduction of phh / tccA2 greatly expanded the catabolic substrate range of pDCA-1. • pDCA-1 had a broad host range including Gram negative and positive strains. • pDCA-1 derivative had great potential in genetic bioaugmentation.
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用类芽孢杆菌对莠去津污染的土壤进行生物强化。
影响因子：
5.2
作者：
Jia W;Li N;Yang T;Dai W;Jiang J;Chen K;Xu X
通讯作者：
Xu X
影响因子：
5.2
作者：
Garbisu C;Garaiyurrebaso O;Epelde L;Grohmann E;Alkorta I
通讯作者：
Alkorta I
影响因子：
4.3
作者：
Shouxiang Wei;Jingjing Miao;Yuhan Li;Yusong Li;Xiufen Wang;L. Pan;Yun Li;Jiang-yue Wu;
通讯作者：
Shouxiang Wei;Jingjing Miao;Yuhan Li;Yusong Li;Xiufen Wang;L. Pan;Yun Li;Jiang-yue Wu;
影响因子：
4.4
作者：
Krol, J. E.;Penrod, J. T.;Top, E. M.
通讯作者：
Top, E. M.
影响因子：
4.1
作者：
Lee, Changsoo;Kim, Jaai;Hwang, Seokhwan
通讯作者：
Hwang, Seokhwan